摘要
目的:研究小檗碱对细胞膜流动性及其膜蛋白荧光的影响.方法:以制备的兔红细胞膜为材料,1,6-diphenyl-1,3,5-hexatriene(DPH)为荧光探针,运用荧光偏振技术检测与小檗碱作用后兔红细胞膜流动性的变化,观察小檗碱与兔血红细胞膜蛋白作用后Tyr残基荧光强度的变化.结果:兔血红细胞膜与小檗碱作用后其荧光偏振P值发生了剂量依赖性的下降;小檗碱使兔血红细胞膜蛋白Tyr残基的荧光产生剂量依赖性的淬灭作用.结论:小檗碱在体外能有效提高细胞膜的流动性,可能与小檗碱多种心血管药理活性有关;小檗碱与膜蛋白的作用可能是膜流动性变化的引发机制.
AIM： To investigate the effects of berberine on the membrane fluidity and membrane protein fluorescence of red-blood cell membrane. METHODS： With rabbit red-blood cell membrane as the experimental material and DPH （ 1,6-diphenyl- 1,3,5-hexatriene） as the fluorescence probe, the effect of berberine on the cell membrane fluidity and membrane protein fluorescence was examined by fluorescence spectrum analytical method. RESULTS ： The P value of rabbit red-blood cell membrane fluidity was decreased and the fluorescence of rabbit red-blood cell membrane protein was quenched by berberine dose-dependently. CONCLUSION： Berberine effectively enhances the membrane fluidity, suggesting that the increasing membrane fluidity is related to the multiple cardiovascular pharmacological activities. The effect of berberine on the membrane protein may result from the changes of membrane fluidity.
出处
《第四军医大学学报》
北大核心
2009年第17期1627-1629,共3页
Journal of the Fourth Military Medical University
关键词
小檗彬药理学
红细胞膜
膜蛋白类
药物生理作用
抗菌作用
berberine/pharmacology
erythrocyte membrane
membrane proteins
physiological effects of drugs
antimicrobial activity
