摘要
目的研究5-氮杂胞苷对大鼠骨髓间充质干细胞(MSCs)增殖及分化过程中延迟整流、瞬时外向和内向整流钾电流(IkDR、Ito、Ik1)的影响。方法按文献方法培养1月龄大鼠MSCs,至第2周时部分MSCs以10μmol/L5-氮杂胞苷(5-Aza)诱导培养4周,以全细胞膜片钳技术检测诱导组第1、2、3和4周IkDR、Ito及Ik1的电流强度,用未诱导组培养6周的检测结果作对照,电流以相应的阻滞剂鉴定。结果各周IkDR、Ito及Ik1检出率相近;诱导组MSCs3种钾电流均随培养时间延长逐渐增强(P<0.05);诱导后第1周MSCs3种钾电流强度与未诱导组比较无明显差异,而从诱导第2周起开始增强(P<0.05),至第4周时进一步增强(P<0.01)。结论5-Aza诱导可促早期MSCs增殖及分化过程中的IkDR、Ito和Ik1增强。
Objective To study the changes of the transient outward K ^＋ current （ Ito）, delayed rectifier K ^＋ current （IkDR） and the inward rectifier K ^＋ current （Ikl） of the rat bone mesenchymal stem cells （MSCs） induced by 5-Azacytidine （5-Aza） during proliferating and differentiating process in vitro. Methods MSCs were cultured according to related articles for two weeks and then some of the cells were induced by 5-Azacytidine. The experiment was divided into uninduced （ cultured for 6w） and induced （ 1,2,3 and 4w） cell groups. Each week had twenty cells random tested by the whole-cell patch clamp technology and the K ^＋ currents were identified by corresponding ionic blockers. Results The detection rate of the same type of K ^＋ current during test weeks had no significant deviation; The three types of K^＋ current intensity were gradually augmented after being inducing cultured for 1,2,3 and 4 weeks （P 〈 0. 05 ）. As compared to the uninduced cells, the same type of K ^＋ current intensity of 1w-induced cells had no significant deviation, however, the current intensity was augmented at 2w-induced cells （P 〈 0. 05 ） and significant augmented at 4w-induced cells（P 〈 0. 01 ）. Conclusion 5-Aza induction showed an early stage augmenting effects to the MSCs induced by 5-Aza during proliferating and differentiating process in vitro.
出处
《基础医学与临床》
CSCD
北大核心
2009年第8期827-830,共4页
Basic and Clinical Medicine
基金
重庆市科委基金([2003]43-92)
