摘要
目的:观察梅毒患者外周血单核细胞(PBMC)表面Toll样受体2、6(TLR2,TLR6)的表达变化及其意义。方法:选取显性梅毒患者87例,采用流式细胞仪检测外周血单核细胞表面TLR2、TLR6蛋白表达水平(荧光强度)以及TLR2阳性细胞率,并与50例健康者作为对照组进行比较。结果:显性梅毒患者组与对照组比较:患者外周血单核细胞TLR2、TLR6的表达水平均明显升高,TLR2高于TLR6,且一期梅毒患者低于二期梅毒患者,差异均有统计学意义(P<0.05);TLR2+CD14+、TLR6+CD14+细胞的阳性率低于对照组,且一期梅毒患者低于二期梅毒患者,差异均有统计学意义(P<0.05);MFI(TLR2)与TLR2+CD14+阳性率、MFI(TLR6)与TLR6+CD14+阳性率,在整个研究对象群体中无明显相关性(r=0.096、r=0.089,P>0.05),但在显性梅毒患者中呈正相关(r=0.813、r=0.752,P<0.05);所有的差异与性别无关,但与年龄有关。结论:梅毒患者TLR2与TLR6的表达显著上调,与病程和免疫状态有关,与性别无关。
Objective： To explore the correlation of toll-like receptor 2 and toll-like receptor 6 expression on the surface of the peripheral blood mononuclear cells of symptomatic syphilitic patients.Methods：The protein expression of TLR2 and TLR6（mean fluorescence intensity,MFI） and the precentage of TLR2^＋CD14^＋ and TLR6^＋CD14^＋ positive cells were evaluated through flow cytometric analysis with peripheral blood monocytes in 87 symptomatic syphilitics and 50 healthy controls.Results：The syphilis dominant group compared with the control group： patients with peripheral blood mononuclear cells TLR2 and TLR6 expression level was significantly increased,and a lower than syphilis patients with secondary syphilis,the differences were statistically significant（P〈0.05）;TLR2^＋CD14^＋ and TLR6^＋CD14^＋ cells in the positive rate is lower than the control group,and a lower than syphilis patients with secondary syphilis,the differences were statistically significant（P〈0.05）.In the entire study,MFI（TLR2） and TLR2^＋CD14^＋ rate、MFI（TLR6） and TLR6^＋CD14^＋ all have no significant correlation（r=0.096、r=0.089,P〉0.05）,but in symptomatic syphilitics group has significant correlation（r=0.813、r=0.752,P〈0.05）.All were unrelated to sex differences,but in the age-related.Conclusion：Syphilis in patients with TLR2 and TLR6 expression increases significantly with the course of disease and immune status,but gender has nothing to do with it.
出处
《中国卫生检验杂志》
CAS
2009年第8期1839-1840,1914,共3页
Chinese Journal of Health Laboratory Technology
关键词
梅毒
TOLL样受体
流式细胞术
Symptomatic syphilitic
Toll-like receptor
Flow cytometry
