摘要
以荷叶为实验材料,用BPCL型微弱发光测量仪,测定了不同浓度NaCl溶液处理不同时间的叶片的延迟发光,观察了延迟发光初始强度随处理时间的变化和延迟发光衰减参数随处理时间的变化。结果表明:随着胁迫时间的增加,0%,0.01%和0.10%处理的叶片,其延迟发光初始强度和衰减参数在一个小范围的上升之后一直呈下降趋势,1.00%,5.00%处理的初始延迟发光强度和衰减参数一直呈下降趋势,10.00%,20.00%处理的初始延迟发光强度和衰减参数从一开始就处于很低的水平。从整体上看,0.10%浓度处理对叶片延迟发光初始强度和衰减参数影响最小。整个实验表明,延迟发光可以规律性地反应盐胁迫对叶片的伤害程度。该结论为生物物理方法测量外界胁迫对叶片的影响具有一定的参考意义。
Delayed luminescence（DL） of bioluminescence is the important content of optic-biology in biophysics.Since 1923, a lot of research has been carried out. In this paper,we choose the lotus leaf as the test materials to experiment. The curve of delayed luminescence with different treating time under different concentrations of NaCl, the changing curve of initial intensity of delayed luminescence with different treating time and the changing curve of attenuation parameters of delayed luminescence with different treating time were researched by BPCL instrument. The results indicated that, with the stress time increasing, the initial strength and attenuation parameters of DL with 0% ,0.01% and 0.10% treatment firstly increased and then decreased; the initial strength and attenuation parameters of DL of 1.00% and 5.00% treatment decreased all along; the initial strength and attenuation parameters of DL of 10.00% and 20.00% treatment decreased to a low-level from the jump; on the whole, 0. 10% NaCl treatment had the lowest effect on the initial strength and attenuation parameters of DL. The experiments showed that the DL can reflect the level of injury of salt stress on leaves. The conclusions of the biophysical methods for measuring the outside world impact of stress on the blades can provide a certain reference value.
出处
《发光学报》
EI
CAS
CSCD
北大核心
2009年第4期545-548,共4页
Chinese Journal of Luminescence
基金
校人才引进项目(2005RC039)资助
关键词
延迟发光
盐胁迫
衰减参数
delayed luminescence
NaCl stress
attenuation parameters
