摘要
[目的]观察活血化瘀方药对Poresky多囊卵巢综合征(PCOS)大鼠模型内皮细胞功能及形态的影响。[方法]依照Poretsky法建立PCOS大鼠模型,随机分为中药组、模型组,并设立正常组作对照。测定各组血清FSH、LH、T、INS及血浆ET、PAI-1的水平,电镜观察内皮细胞形态及超微结构变化。[结果]模型组内皮细胞间的连接失去正常形态,基底膜不连续,细胞膜损伤、不完整;胞质内空泡明显增多,线粒体膨胀,嵴溶解、断裂、消失;细胞核聚集,异染色质增多。中药组主动脉内皮细胞间连接较紧密,线粒体无明显肿胀,嵴清晰,吞饮泡和溶酶体增多,胞质偶见空泡。模型组血浆PAI-1、ET比正常组增高,差异有显著性(分别为P<0.01,P<0.05),中药组与模型组比较,ET、PAI-1降低(P<0.05);与正常组比较PAI-1水平无显著差异,ET增高(P<0.05)。[结论]PCOS大鼠模型存在内皮细胞形态、超微结构改变,血浆ET、PAI-1升高,提示PCOS存在血瘀的微观病理基础。活血化瘀中药能够从形态学上修复PCOS大鼠损伤的内皮细胞,调整内皮细胞功能。
[Objective]To observe the effect of Chinese herbs for activating blood stasis on the shape and function of the endothelial cells in rat aorta. [Method]Rat models of PCOS have been established according to Poresky, randomized into animal model and Chi nese medicine group（TCM group）, normal rats were control group. Measure the serum level of FSH, LH, T, INS and plasma ET PAI-1, and observe the endothelial cell morph. [Results] In the model group,the endothelia cells of the aorta were contracted, with discontinuous basement membrane, inflated chondriosome, the middle layer smooth muscles tending to move inside and out of the normal shape of intraendothelial junction. In the TCM group, the endothelial cells appeared flat, arranging along the long axis of the blood vessels, normal endothelial cell integrity with scanty cytoplasm and the microscopic appearance shows highly developed golgiosome rough endoplasmic reticulum and smooth endoplasmic reticulum. The model group had a more increased serum level of PAII and ET than those of the control group, and showed the significant difference （P〈0.01 ,P〈0.05）. The TCM group had a significantly lower serum level of PAI-1 and ET than those of the model group（P〈0.05）. There were no significant differences between the normal group and the TCM group on serum level of PAI-1（P〉0.05）. The TCM group had more decreased level of ET than those of the normal group（P〈0.05）. [Conclusion] The morphologic changes of the endothelial cells and increase of ET and PAI-1 were found in the PCOS model rat,indicating blood stasis was one pathogenesis of PCOS. Chinese herbs for activating blood stasis could morphologically repair damaged endothelial cells, regulate their functions.
出处
《浙江中医药大学学报》
CAS
2009年第4期483-485,共3页
Journal of Zhejiang Chinese Medical University
