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临床分离多重耐药肠杆菌科细菌Ⅰ类整合子检测及耐药性研究 被引量:4

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摘要 目的检测临床分离多重耐药肠杆菌科细菌Ⅰ类整合子分布,分析Ⅰ类整合子在细菌多重耐药中的作用。方法标准纸片扩散法(Kirby-Bauer test)检测耐药性,PCR方法扩增Ⅰ类整合酶基因,经电泳后检测扩增产物。结果在分离出的408株细菌中,共有246株检出Ⅰ类整合子,总体检出率为60.3%(246/408),其中大肠埃希菌检出率为62.4%(126/202),肺炎克雷伯菌检出率为56.8%(71/125),阴沟肠肝菌检出率为60.5%(49/81)。整合子阳性株对多种抗生素的耐药率明显高于整合子阴性株,亚胺培南对所有菌株敏感。结论Ⅰ类整合子对细菌多重耐药性的产生和传播起着重要作用,应加强临床细菌基因水平的耐药监测,采取有效措施防止耐药性的传播。
出处 《广东医学》 CAS CSCD 北大核心 2009年第7期1105-1106,共2页 Guangdong Medical Journal
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