摘要
以50%乙腈超声提取样品后,再以乙腈-0.02 mol/L乙酸铵溶液(含0.1%甲酸)为流动相,经C18柱分离后,ESI源离子化,然后采用正离子模式定性判别苯乙双胍、格列吡嗪、格列本脲,据此建立了降糖类中成药中非法添加降糖化学成分的快速液质定性方法。此方法检测快速灵敏、选择性强,适用于大批量样品的定性鉴别。
To establish a HPLC/MS/MS method for simultaneous qualitative screening hypoglycemic agents, which were illegally added to proprietary Chinese medicine and health food. Samples were ultrasonic extraction by 50% ACN and then separated by a C18 column with the mobile phase of 0.02 mol/L ammonium acetate （0.1% formic acid） and ACN. Hypoglycemic agents in sample were as certained as compared with the mass spectrogram of samples and reference substance, which gained by electrospray ionization tandem mass in positive ionization model. It is approved the method is rapidly detection with high selectivity and sensitivity.
出处
《应用化工》
CAS
CSCD
2009年第5期740-743,共4页
Applied Chemical Industry
基金
国家自然科学基金(20877038)
湖南省卫生厅科研项目(B2006-103)
关键词
降糖类中成药
化学成分
定性分析
液质联用
hypoglycemic Chinese patent drugs
chemical constituents
qualitative analysis
tandem mass
