摘要
A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented.Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels ]via] photopolymerization in a polyacrylamide matrix.The resulting polymeric,hydrogel plugs are porous under electrophoretic conditions,and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA.The total analysis process can be completed within 5 min,and the limit of detection is 0.1 μmol/L.This method is simple,rapid and feasible.The double-stranded DNA can be chemically denatured,and the chip is reusable.The conditions for photopolymerization,hybridization,and denaturation were discussed as well.
A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels v/a photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0. 1 μmol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2009年第6期1128-1130,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20635030,20575067)
科技部“八六三”计划(批准号:2006AA02Z305)资助
