摘要
利用循环伏安法（ＣＶ）研究了神经递质多巴胺（ＤＡ）、肾上腺素（Ｅｐ）及去甲肾上腺素（ＮＥ）在聚中性红膜修饰电极（ＰＮＲＥ）上的电化学行为。根据ＤＡ及ＥＰ在ＰＮＲＥ上还原过程的差异，采用阴极导数伏安法，实现了ＤＡ及Ｅｐ的同时测定．在测定的最佳条件下，ＤＡ及Ｅｐ在ＰＮＲＥ上的还原峰电流与其浓度分别在：１．０ × １０－６１．８×１０－４ｍｏｌ／Ｌ及４．５×１０－７～４．５×１０－６ｍｏｌ／Ｌ范围内具有良好的线性关系．由于抗坏血酸（ＡＡ）在ＰＮＲＥ上的电催化氧化是不可逆的，因而基本消除了ＡＡ对ＤＡ及Ｅｐ测定的干扰。
The electrochemical behavior of neurotransmitters, such as dopamin (DA ). epinephrine(Ep) and norepinephrine, in poly(neutral red) film modified electaodes (PNRE) have been studied by cyclic voltammetry. According to the discrepancy of reduction process of DA and EP in PNRE, the simultaneous determination of DA and Ep has been realized with cathode derivative voltammetry. Under the selected conditions, the reduction peak currents are in linear relationship with both DA and EP concentrations in the ranges of 1. O×10-6-l. 8 × 10-4 mol/L and 4. 5 × 10-7- 4. 5 × 10-6 mol/L, respectively. Because the oxidation of ascorbic acid(AA) is an irreversible reaction in PNRE, as a result the interferences of AA for determining DA and Ep were eliminated basically.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第5期506-510,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题
关键词
神经递质
多巴胺
肾上腺素
去甲肾上腺素
分析
Poly (neutral red ), neurotransmitters, cyclic voltammetry, cathode derivative voltammetry, chemically modified electrodes
