摘要
目的:探讨利用荧光染料CFSE标记法及MTT法,观察人NK、T混合淋巴细胞(NKTm)体外杀伤MDA-MB-231肿瘤株的效果。方法:应用CFSE/PI染色法及MTT法标记人乳腺癌MDA-MB-231肿瘤株,观察NKT细胞联合顺铂在各种时序下对乳腺癌MDA-MB-231肿瘤株的杀伤效果。结果:CFSE染色条件下,人NKTm细胞体外对MDA-MB-231细胞效靶比E/T=25:1时较E/T=12.5:1时杀伤率明显升高;E/T=12.5:1时杀伤率略低于顺铂;先加入NKTm各组随与顺铂间隔时间的延长而杀伤率升高(以上P均<0.05)。结论:NKTm细胞对MDA-MB-231肿瘤细胞的直接杀伤作用强,同顺铂联用时要提高对肿瘤细胞的杀伤率应先给予NKTm细胞后应用顺铂。
Objective： To investigate the combined effects of NK and T lymphocytes （ NKTm cells ） and cisplatin （ DDP ） on CFSE labeled human breast cancer cell line MDA-MB-231. Methods： CFSE/PI labeled cell line MDA-MB-231 was treated with NKTm ceils or with NKTm ceils and cisplatin. Cytotoxic activity was determined by MTT colorimetric assay and flow cytometry with specific antibodies. Results： The NKTm cells showed their cytotoxic effects on CFSE labeled cell line MDA-MB-231 in a concentration- dependent manner（P〈0.05 ） ,The inhibition rate of NKTm cells （ effector/target= 12.5：1 ） on CFSE labeled cell line MDA-MB-231 was lower than that of cisplatin （P〈0.05）. A synergistic effect of NKTm and cisplatin on CFSE labeled ceil line MDA-MB-231 was also observed, The inhibition rate was higher in groups that received NKTm before cisplatin than in groups that received NKTm after cisplatin （ P〈0.05 ）. IN groups that received NKTm first, the inhibition rate increased, but did not increase in groups that received cisplatin first, as demonstrated by MTT assay. Conclusion： NKTm ceils have a highly direct cytotoxic effect on breast cancer cell line MDA-MB-231. NKTm cells and cisplatin have a synergetic effect on CFSE labeled cell line MDA-MB-231.
出处
《军医进修学院学报》
CAS
2009年第3期378-379,385,共3页
Academic Journal of Pla Postgraduate Medical School
