摘要
目的研究伽玛刀照射猫脑组织在不同时期产生的病理学变化。材料与方法３３只家猫分成１１组，１０个实验组分别用１０，２０，３０，４０，５０，７０，１００，１６０，１８０，２００Ｇｙ的剂量照射其大脑皮质，照射后３月、半年和１年（≥１６０Ｇｙ在１月、３月和半年）光镜观察组织学变化。结果（１）≥１６０Ｇｙ均引起局限性脑组织坏死灶，半年时坏死灶缩小；（２）１００Ｇｙ和７０Ｇｙ３月时未见坏死灶，１年时神经细胞坏死消失，残留者固缩；（３）５０Ｇｙ３月无变化，半年和１年引起神经元变性和固缩。４０Ｇｙ半年无变化，１年时神经元变性；（４）３０Ｇｙ１年时仅局部细胞数减少，２０和１０Ｇｙ无变化。结论（１）≥１６０Ｇｙ引起局限性脑坏死，２００Ｇｙ产生的坏死灶较大；（２）１００Ｇｙ和７０Ｇｙ引起早期迟发放射反应和严重的晚期反应；（３）４０Ｇｙ和５０Ｇｙ引起晚期迟发放射反应。
Purpose To investigate the pathological changes of the brain irradiated by gamma knife(GK).Materials and Methods Thirtythree cats were divided into 11 groups(n=3),and the cerebral cortex of cats in experimental groups were irradiated with doses of 10,20,30,40,50,70,100,160,180 and 200 Gy respectively.After GK,one animal in each highdose group(≥160Gy) was killed at 1,3 and 6 months respectively,and one animal in low and median dose groups was sacrificed respectively at 3,6 and 12 months.Histopathological changes were examined by light microscope with HE,Luxol fast blue and GFAP.Results (1) A circumscribed cerebral necrosis were seen with necrotic region shrinking with time after 160,180 and 200 Gy irradiation.(2) Degenerative and shrank neurons presented without necrosis three months in 70 and 100 Gy groups and the number of neurons decreased significantly and the remainders contracted or necrosed in 12 months.(3) Cats irradiated with 50 Gy showed a few degenerative neurons in 6 months and karyopyknosis was obvious with mononuclear infiltration in one year;40 Gy caused reduced number of neurons in small local cortex and some neurons degeneration was observed in 12 months.(4) only reduced number of neurons in very small region was observed in 30 Gy group and there was no histopathological change found in cats with 20 and 10 Gy. Conclusion (1) 200 Gy produces larger necrosis as compared to 160 Gy.(2) 100 and 70 Gy result in early delayed and serious late radiobiological reaction.(3) 50 and 40 Gy induces late radiobiological effects.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
1998年第1期29-32,共4页
Chinese Journal of Radiation Oncology
