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Ectoine提高啤酒麦芽中酶的热稳定性的研究 被引量:5

Research on the Improvement of the Thermostability of Enzyme in Malt by Ectoine
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摘要 研究了Ectoine对啤酒麦芽中淀粉酶、β-葡聚糖酶和纤维素酶热稳定性的影响。在不同浓度的Ectoine存在下,考察比较热处理后的上述3种酶的残余酶活性。结果表明,经80℃和90℃热处理10min后,与未添加Ec-toine的相比,添加5.0mmol Eectoine,淀粉酶残余酶活力分别提高了17.6%和26.8%;添加3.0mmol的Ectoine,β-葡聚糖酶残余酶活力分别提高了25.7%和34.6%;添加3.0mmol的Ectoine,纤维素酶残余酶活力分别提高57.6%和50.8%。 The effects of the addition of Ectoine on the thermostability of amylase, β-glucanase and cellulase in malt were studied. The residual enzyme activities of the above three kinds of enzyme after heat treatment were investigated in the presence of different Ectoine content. The results showed that after 10 min heat treatment at 80 ℃ and at 90 ℃, the addition of 5.0 mmol Ectoine could improve the residual amylase activities by 17.6 % and by 26.8 % respectively (compared with that without ectoine addition), the addition of 3.0 mmol Ectoine could improve the residual β-glucanase activities by 25.7 % and by 34.6 % respectively, and the addition of 3.0 mmol Ectoine could improve the residual cellulase activities by 57.6 % and by 50.8 % respectively.
作者 郑昕 张苓花
出处 《酿酒科技》 2009年第5期36-38,共3页 Liquor-Making Science & Technology
基金 国家自然科学基金项目(20776021)
关键词 啤酒生产 ECTOINE 麦芽 淀粉酶 Β-葡聚糖酶 纤维素酶 热稳定性 beer Ectoine malt amylase β-glucanase cellulase thermostability
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参考文献11

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共引文献33

同被引文献33

  • 1李德转.甘啤3号啤酒大麦质量及应用现状分析[J].酿酒科技,2004(4):63-64. 被引量:2
  • 2舒志全,罗昭锋,张毅,瞿鑫,时青,刘忠,何立群,高大勇.红细胞在保护剂添加和洗涤时的非平衡渗透响应实验研究[J].中国生物医学工程学报,2006,25(1):25-29. 被引量:4
  • 3李强,王越,王运吉,张苓花.Ectoine对Cu-Zn型SOD酶热稳定性的影响[J].大连轻工业学院学报,2007,26(2):116-119. 被引量:3
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  • 8KATRIN G, ANGLEA V, HANS J K. New Type of Osmoregulated Solute Transporter Identified in Halophilic Members of the Bacteria Domain: TRAP Transporter TeaABC Mediates Uptake of Ectione and Hydroxy Ectione in Halomonas elongata DSM 258IT[J]. Journal Of Bacteriology,2002,184:3078-3085.
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