摘要
通过改良热裂解法提取沙门氏菌基因组DNA,设计特异性引物进行PCR扩增,并对热裂解的时间进行了比较试验,对PCR检测的敏感性和所用引物的特异性进行了试验。结果表明,煮沸时间为2min即可获得满足PCR要求的基因组DNA;设计的引物特异性好,能专一性扩增出约500bp条带;该引物灵敏度高,能进行有效检测的核酸最低起始量为129pg。采用热裂解法提取沙门氏菌基因组DNA进行PCR快速检测技术大大缩短了沙门氏菌检测时间。
The pyrolysis was selected to obtain the genome DNA of Salmonella, and the time of pyrolysis, the sensitivity of PCR to the template DNA and the specificity of primer were studied. The result showed that the good effectiveness of PCR was obtained when the mycelium of Salmonella was treated by boiling for 2min; the primers used in this experiment was specific to Salmonella and the band of 500bp was obtained the 129pg DNA of Salmonella would be able to detect by PCR. The detection time of Salmonella was shortened when PCR detection technology was adopted and the pyrolysis was selected to obtain the genome DNA for PCR and this detection technology would service the immigration food safety testing better.
出处
《湖北农业科学》
北大核心
2009年第3期527-529,共3页
Hubei Agricultural Sciences
基金
广东省自然科学基金项目(B5041413)
关键词
沙门氏菌
PCR
快速检验
Salmonella
polymerase chain reaction （PCR）
rapid detection
