摘要
为了研究转基因小麦QQ5沉默的HMW-GS基因的遗传规律,以QQ5与育成品种高原314和新春13号进行正反交,再用育成品种进行回交,采用SDS-PAGE技术检测并分析各组合亲本、F1、F2、BC1F1的HMW-GS组成。结果表明,HMW-GS基因沉默表现为显性,且能稳定遗传,杂交及回交后代符合3∶1和1∶1的分离比,遵循孟德尔遗传方式。
All the high-molecular-weight glutenin subunits （HMW-GS） were suppressed to express in transgene-induced gene silencing wheat QQS. Crosses and backcrosses between transgenic wheat line QQ5 and two elite wheat varieties have been made to study the inheritance and stability of silenced HMW-GS genes. The two wheat （Triticum aestivum L. ） varieties were Plateau 314 and Xinchun 13 with high yield and good adaptability in northwestern China. Different crosses and generation populations were obtained. The grains of parents, F1, F2 and backcross progenies BC1F1 were analyzed by SDS-PAGE method for HMW-GS genes. The results showed that the phenomenon of HMW-GS gene silencing was dominantly controlled by one functional gene and inherited stably in progenies according to the Mendelian pattern. The stable HMW-GS silenced lines would be beneficial to weak wheat breeding.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2009年第2期185-188,共4页
Journal of Triticeae Crops
基金
中国科学院知识创新工程重要方向项目(KSCX2-YW-N-052)
国家科技支撑计划项目(2007BAD52B08)
中国科学院知识创新工程重大项目(KSCX1-YW-03)
青海省重点科技攻关项目(2006-N-137)
