摘要
目的:建立测定啤酒中微量甲醛的新方法。方法:采用蒸馏法将啤酒中微量甲醛分离出来,甲醛与酚试剂反应生成吖嗪,吖嗪在酸性溶液中被高铁离子氧化形成蓝绿色化合物,根据颜色深浅,比色测定。结果:线性范围在0.0～3.2 mg/L,相关系数r=0.9998;相对标准偏差≤4.3%;加标回收率在91.3%～96.9%;在本文实验条件下,二氧化硫等干扰得以消除,样品测定结果与国标方法测定结果的差异无显著性。结论:该法操作简单,灵敏度高,定量准确,适合啤酒中微量甲醛的测定。
Objective： To establish a new method for determination of trace formaldehyde in beer. Methods： Formaldehyde was dissociated from beer via distillation method, and then reacted with phenol reagent to obtain azine. Aziue was oxidized by high - valence iron ions to form blue - green compounds in acid solution. Colorimetric determination was carried out according to the color differences. Results：The method was proved to be linear in the range of 0. 0 ~ 3.2 mg/L,linear regression coefficient was equal to 0. 9998, relative standard deviation was no more than 4. 3% , and standard - weight recoveries were between 91.3 % and 96. 9%. Interferences from sulfur dioxide and so on were eliminated in the test. The results were similar to those from national standard method. Conclusion： The method is simple, sensitive and accurate and it is suitable for detection of trace formaldehyde in beer.
出处
《中国卫生检验杂志》
CAS
2009年第1期101-102,共2页
Chinese Journal of Health Laboratory Technology
关键词
酚试剂
分光光度法
啤酒
甲醛
Phenol reagent
Spectrophotometer
Beer
Formaldehyde
