摘要
分析了大麦黄花叶病毒的外壳蛋白及基因组ＲＮＡ的性状。ＳＤＳ－聚丙烯酰胺凝胶电泳表明，该病毒的外壳蛋白由单种亚基组成，其分子量为３４．９ＫＤａ，在常规提纯过程中，该病毒的外壳蛋白较不稳定，部分易降解为２８．０ＫＤａ和２６．６ＫＤａ的组分；不同毒源病毒的外壳蛋白分子量无明显差异，但其稳定性差异较大。１．２％变性琼脂糖凝胶电泳的测定结果表明，大麦黄花叶病毒的基因组ＲＮＡ由二组分组成，其大小分别为７．７８Ｋｂ（ＲＮＡ－１）和３．４８Ｋｂ（ＲＮＡ－２），且不同毒源间无明显差异。以Ｏｌｉｇｏ（ｄＴ）１５为引物反转录合成ｃＤＮＡ的实验表明，ＲＮＡ－１和ＲＮＡ－２的３端均带ｐｏｌｙ（Ａ）的结构，与国外已报道的相符。
The properties of capsid proteins and genomic RNAs of barley yellow mosaic virus (BaYMV) collected from Yangzhou, Yancheng,Rudong and Shanghai were analysed.The result of SDS PAGE showed that the capsid protein subunit of BaYMV consisted of one component with MW 34 9 KDa,unstable and degraded easily into two components with 28 0 KDa and 26 6 KDa in process of normal purification.The capsid protein subunit of BaYMV from different areas had almost identical molecular weight, but different stability.Agarose gel electrophoresis test demonstrated that there were two components with 7 78 Kb (RNA 1) and 3 48 Kb (RNA 2) in the genomic RNAs,and no difference was found among viruses from different sources.The result of reverse transcription using Oligo(dT) 15 as a primer showed that both RNA 1 and RNA 2 contained poly(A) tail at 3 terminals as reported before.
出处
《江苏农业学报》
CSCD
北大核心
1998年第1期27-30,共4页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金
江苏省自然科学基金
江苏省青年基金
