摘要
利用３株特异性单抗，建立了检测ＥＤＳＶ的夹心ＥＬＩＳＡ方法。经测定，ＭｃＡｂ最适包被浓度为２μｇ／ｍｌ，ＭｃＡｂ－ＨＲＰ最佳工作浓度为１∶４００，该方法最小检出浓度为０．０３μｇ／ｍｌ，与常见的几种鸡的传染病的病原无交叉反应。对某发病鸡场的５０份泄殖腔拭子样本进行了检测，阳性率为８４％（４２／５０）。
Sandwich enzyme linked immunosorbent assay(ELISA) for detecting Egg Drop Syndrome virus was developed by using three strains mono clonal anbibodies (McAbs).The optimal coating concentration of McAbs was 2μg/ml Working concentration of McAb HRP was 1∶400.The sensitivity of the McAb sandwich ELISA was 0.03μg/ml.The assay had no cross reaction with common pathogenic microorganisms.50 cloaca swab samples from infected birds were detected,resulting in positive rate of 84%(42/50).
出处
《中国兽医杂志》
CAS
北大核心
1998年第3期8-9,共2页
Chinese Journal of Veterinary Medicine
