摘要
蛇胸腺是一种神经内分泌器官，为了从系统演化的角度探讨胸腺和其他免疫器官的内分泌特性，应用显示ＡＰＵＤ细胞的组织化学方法和免疫组织化学方法对昆明系小鼠（Ｍｕｓｍｕｓｃｕｌｕｓ）、Ｗｉｓｔａｒ大鼠（Ｒａｔｕｓｒａｔｕｓ）、豚鼠（Ｃａｖｉａｐｏｒｃｅｌｕｓ）、家鸡（Ｇａｌｕｓｇａｌｕｓｄｏｍｅｓｔｉｃａ）、北京鸭（Ａｎａｓｐｌａｔｙｒｈｙｎｃｈｏｓｄｏｍｅｓｔｉｃａ）、中华大蟾蜍（Ｂｕｆｏｂｕｆｏｇａｒｇａｒｉｚａｎｓ）、黑斑蛙（Ｒａｎａｎｉｇｒｏｍａｃｕｌａｔａ）、非洲爪蟾（Ｘｅｎｏｐｕｓｌａｅｖｉｓ）、北方狭口蛙（Ｋａｌｏｕｌａｂｏｒｅａｌｉｓ）、大鲵（Ａｎｄｒｉａｓｄａｖｉｄｉａｎｕｓ）和鲫鱼（Ｃａｒａｓｉｕｓａｕｒａｔｕｓ）共１１种脊椎动物进行了勘定。结果表明仅无尾两栖类和鸟类与虎斑颈槽蛇（Ｒｈａｂｄｏｐｈｉｓｔｉｇｒｉｎａ）相同，胸腺含有少量嗜银细胞、亲银细胞、铅苏木精阳性细胞和多种神经内分泌激素免疫组化阳性细胞，而其他免疫器官，尤其是腔上囊均为阴性。不同动物胸腺的免疫组化反应具有种间差异。结合对蛇的研究，可以看出胸腺确实具有一定的神经内分泌功能，是一内分泌器官。
お? According to the previous studies,snake (Rhabdohpis tigrina) thymus is also a neuroendocrine organ The existence of APUD cells in the immune organs and their properties were observed in 11 species of different vertebrate groups from fish to mammal with special staining techniques and immunocytochemical methods in this paper The results showed that there were some argyrophil,argentaffin,lead-haemutoxylin positive and 5-HT,Gas or SS-immunostaining cells in thymus of frogs (Rana nigromaculata,Kaloula borealis),toads (Bufo bufo gargarizans,Xenopus laevis),chicken (Gallus gallus domestica) and Peking duck (Anas platyrhynchos domestica) This is the same as in the snake,but in the other immune organs According to the immunostaining result,there was a difference in type and number of positive cell between the species The result indicated that thymus had neuroendocrine function and was a neuroendocrine organ from the evolutionary point
基金
国家自然科学基金
山东省自然科学基金
关键词
脊椎动物
免疫器官
胸腺
APUD细胞
Vertebrate,Thymus,APUD cell,Neuroendocrine cell immunocytoch-emistry
