摘要
目的用实时荧光定量聚合酶链反应(FQ-PCR)技术准确度定量检测江门市区凝有非淋菌性尿道炎患者沙眼衣原体(CT)、解脲支原体(UU)基因,进一步了解UU、CT的感染情况,为临床治疗提供依据。方法运用实时荧光定量PCR法(FQ-PCR)对1501例凝有非淋菌性尿道炎患者同时进行CT、UU检测。结果CT的阳性率是13.1%,UU的阳性率是33.9%,二者之间阳性率比较有显著性差异。结论1.江门市区非淋菌性尿道炎患者病原体CT、UU中占有一定比例,二者之间感染率比较有显著性差异。2.荧光实时定量PCR检测UU、CT具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点,其结果对临床诊断、治疗有一定指导意义。
Objective： To detect and analysis chlamydia trachomatis and ureaplasm urealyticum infection in non -gonococcal urethritis by fluorescence quantitative PCR assay in Jiangmen city. Methods ： The chlamydia trachomatis and ureaplasm urealyticum of non - gonococcal urethritis by fluorescence quantitative PCR assay. Results： The positive rete of CT were 13.1%, and the positive rete of UU were 33.9%, The positive rete were respective . The mixed infect were rate in the group. Conclusion： 1. CT and UU infection play a certain role in non -gonococcal urethritis and physician should pay attention for it . 2. The fluorescence quantitative PCR as- says showed advantages which were mainpulated simply, time - saving, more extra, more sensitive and more spectific in detecting CT and UU. FQ - PCR had directive meaning for dagnosis and treatmeat.
出处
《中国优生与遗传杂志》
2008年第12期38-39,共2页
Chinese Journal of Birth Health & Heredity
