摘要
利用苯乙烯单封端的聚醋酸乙烯酯(PVAc)大分子单体与苯乙烯进行分散共聚,制得了表面光滑、直径在500～700 nm之间、具有较好单分散性的聚苯乙烯接枝PVAc(PSt-g-PVAc)核壳结构复合微球.在碱性条件下醇解得到PSt-g-PVA微球,进而由活性染料汽巴蓝(CB)与微球表面的羟基进行亲核反应,制得了汽巴蓝功能微球(CB微球).经元素分析测定得到CB微球中CB的含量为139.22μmol/g.该CB微球对人血清白蛋白(HSA)的吸附量为40.9 mg/g.由硫氰酸钠进行解吸试验,吸附在微球上的HSA的解吸率为92.11%,且CB微球的重复使用性能良好.
The PSt-g-PVAc microspheres were obtained by dispersion copotymerization of styrene （St） and PVAc macromonomer with a vinylbenzyl end group. And diameters of the PSt-g-PVAc microspheres are 500-700 nm. PSt-g-PVA composite microspheres were derived from alkaline hydrolysis from PSt-g-PVAc microspheres. The functional microspheres were prepared via the nucleophilic reaction between PSt-g-PVA microspheres and Cibacron Blue F3GA （CB）. The biggest amount of CB on surfaces of the functional microspheres was 139.22 μmol/g which measured by using an elemental analysis instrument. The amount of HSA adsorption on functional microspheres was 40.9 mg/g. The highest desorption ratio （92.11%） was achieved by using an eluent of 0.5 mol/L NaSCN （pH 8.0）. The functional microspheres could be reused without significant decreases in the adsorption capacities.
出处
《天津工业大学学报》
CAS
2008年第5期19-22,共4页
Journal of Tiangong University
基金
国家自然科学基金(20671043)
江苏高等学校优秀科技创新团队资助项目(苏教产[2007]5号)
关键词
大分子单体
汽巴蓝
功能微球
人血清白蛋白
吸附
macromonomer
Cibacron Blue
functional microsphere
human serum albumin
adsorption
