摘要
[目的]了解病毒性脑炎病例中肠道病毒感染的比例及流行型别分布,积累病原学诊断经验。[方法]采集2004至2007年监测病例脑脊液标本,细胞培养分离肠道病毒,RT-PCR诊断,组合血清中和试验与EVVP1基因序列分析进行血清型别鉴定。[结果]246例标本肠道病毒分离阳性率19.5%,病毒分离与RT-PCR检测总阳性率34.5%;流行/暴发病例分离阳性率(41.2%)高于一般住院病例(13.8%);阳性率呈逐年下降趋势(44.7%～2.5%)。分离到的均为埃坷病毒(echovirus,ECV),其中ECV30型占43.8%。RT-PCR诊断阳性率(49.0%)显著高于病毒分离率(28.9%)。[结论]病毒性脑炎中肠道病毒占比例高,ECV30为常见血清型;RT-PCR诊断敏感、快速,有实际应用价值。
[Objective] To study the infection rate and the epidemiological sero-types of enterovirus （EV） for the pathogenic diagnosis of viral encephalitis cases. [Methods] Virus isolation and RT-PCR essay were employed to detect EV in cerebrospinal fluid （CSF） collected from the patients diagnosed as viral encephalitis by clinical feature from 2004 to 2007. Typing the serotype of EV by sero-neutralization test and/or analysis of sequence of VP1 gene. [Results] In 246 CSF specimens,virus positive rate was 19.5% （48/246）. Totally positive rate was 34.6% （85/246）, tested by EV positive or RT-PCR positive. The positive rate of CSF collected from epidemic or outbreak cases （41.2%） was higher than normal hospitalized patients （13.8%）. Positive rate showed decreasing trend （from 44.7% to 2.5%）. The sero-types of isolated virus were echovirus （ECV） and 43.5% for the type ECV30. RT-PCR positive rate was more sensitive （49.0%） than virus isolation （28.9 %）. [Conclusion] Viral encephalitis cases result mostly from EV and ECV30 type is the preponderant sero-type. RT-PCR is a rapid and sensitive method for detecting the pathogens of viral encephalitis.
出处
《海峡预防医学杂志》
CAS
2008年第4期1-3,共3页
Strait Journal of Preventive Medicine
基金
福建省自然科学基金计划项目(项目号:2006J0081)
福建省科技重大专项(项目号:2004Y201-2)
关键词
病毒性脑炎
肠道病毒
逆转录聚合酶链反应
Viral Encephalitis
Enterovirus （EV）
Reverse Transcription
Polumerase Chain Reaction （RT-PCR）
