摘要
【目的】从辣椒花药中克隆与胚状体发育相关的基因,并对其进行序列分析。【方法】以LTP基因、GST基因同源序列设计简并引物,采用RT-PCR方法对辣椒小孢子胚状体发育相关基因进行克隆,测序后对其进行分析。【结果】获得2个可能与辣椒花药胚状体发育相关的基因片段PELTP(GenBank登录号为:EF583618)和PEGST(GenBank登录号为:EF583619),其长度分别约为750 bp和1 000 bp。序列分析表明,PELTP基因与辣椒LTP基因同源性为98%,PEGST基因与辣椒GST基因的同源性为99%。【结论】PELTP和PEGST基因可能在辣椒小孢子胚状体发育早期起着重要作用。
[Objective] The objective of this study was to clone embryogenesis related gene from pepper anther culture and cnalyze their sequences. [Method] Homologous sequence amplification method was used in gene isolation. The RT-PCR method was used to identify differential gene expression in the process of microspore-derived embryogenesis in pepper. [Result]Two gene sequences PELTP （accession number： EF583618） and PEGST（accession number：EF583619） were obtained,which proved to be expressed at the embryoids. The sequence lengths were 750 bp and 1 000 bp respectively. Blast results showed that PELTP was highly homologous to LTP gene of pepper and PEGST to GST gene of pepper. Homology was 98% and 99% respectively. [Conclusion] It is presumed that the two genes may play an important role in the early stages of pepper microspore embryogenesis.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2008年第9期123-127,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(30771467)
国家"十一五"科技支撑计划项目(2006BAD01A7)
西北农林科技大学植物育种专项项目(05YZ024-1)
关键词
辣椒
花药培养
胚状体
基因克隆
pepper（Capsicum annuum L）
anther culture
embryoid
gene cloning
