摘要
目的通过检测Caspase活性分析si RNA对宫颈癌CaSki细胞凋亡的影响,评价si RNA的生物学效应。方法应用FuGene6为转染试剂,将si RNA转染入CaSki细胞,采用Western blotting检测细胞提取物中HPV16-E6的表达;用Caspase-3,-8,-9Colori metric Assay Kit试剂盒检测Caspase活性。结果si RNA抑制HPV16-E6表达;可活化Caspase-3,-8和-9。结论检测Caspase活性,能评价si RNA对宫颈癌CaSki细胞凋亡的影响。
Objective To analyze the effects of siRNA on apoptosis of cervical cancer CaSki cell through detecting Caspase activity, and to evaluate the biological effect of siRNA. Methods HPV16 - E6 siRNA was transfected into CaSki cell with FuGene 6, and protein HPVl6- E6 was detected by Western blotting. The activity of Caspase was determined by Caspase- 3, - 8, and - 9 Colorimetric Assay Kit. Results siRNA inhibited HPV16 - E6 expression and activated Caspase- 3, - 8, and - 9. Conclusions Detecting Caspase activity can evaluate the effects of siRNA on apoptosis of cervical cancer CaSki cell.
出处
《实用预防医学》
CAS
2008年第4期975-976,共2页
Practical Preventive Medicine
基金
湖南省自然科学基金(07JJ8058)
