摘要
目的观察hVEGF165在自体骨髓间充质干细胞移植治疗兔缺血后肢中的表达。方法体外培养兔骨髓间充质干细胞,通过腺病毒载体转染hVEGF165基因。移植后4周内,采用RT-PCR法鉴定hVEGF165在兔缺血后肢中的表达。结果hVEGF165成功转染骨髓间充质干细胞,移植后在兔缺血后肢中有效表达。结论hVEGF165转染干细胞后能在体内有效表达。
Objective To observe the expression of Vascular endothelial growth factor 165 （VEGF165 ） in transplantation of MSCs to repair an ischemically damaged bind limb in a rabbit model. Methods MSCs were cultivated in vitro and transfected with non - replicative adenoviral vector with VEGF165 gene. Four weeks after treatment, Real - time PCR assessed the expression of VEGF165 in the damaged limb. Results It was confirmed that VEGF165 was successfully transfected into MSCs. Real - time PCR assessments revealed the expression of Vascular endothelial growth factor 165 （ VEGF165 ） in 4 weeks after treatment. Conclusion VEGF165 can be transfected into MSCs and express in rabbit model of ischemic limb.
出处
《医学研究杂志》
2008年第6期39-41,共3页
Journal of Medical Research
基金
浙江省金华市科学技术计划研究项目(金市科字[2007]45号
计划编号:2007-3-017)
