摘要
目的研究银耳孢糖(Tremella fuciformis spores polysaccharide,TSP)对小鼠移植性肿瘤及免疫功能的影响。方法利用小鼠肝癌H22及小鼠肉瘤S180的体内移植性肿瘤模型,腹腔连续给予TSP12d,称小鼠体重及肿瘤、脾脏和胸腺重量,测定脾淋巴细胞转化率及NK细胞杀伤活性。同时观察TSP对正常小鼠免疫器官重量和碳粒廓清的影响。结果TSP25,50,100mg·kg-1对小鼠肝癌H22及小鼠肉瘤S180有一定的抑制作用,抑瘤率分别为17.8%,27.6%,34.9%;32.8%,36.1%,44.0%;TSP50,100mg·kg-1能提高荷瘤小鼠脾NK细胞杀伤活性及淋巴细胞转化率(P<0.05)。此外,TSP50,100mg·kg-1亦能明显提高正常小鼠脾脏和胸腺的重量及网状内皮系统吞噬功能(P<0.05)。结论TSP对小鼠移植性肿瘤有一定的对抗作用,其抗肿瘤作用可能与增强机体免疫能力有关。
OBJECTIVE To study the effect of Tremella fuciformis spores Polysacchafides （TSP） on transplanated tumor and the immunological function in mice. METHODS The transplanted tumor model with Hepatoma-22 and Sarcoma 180 were established for anti-tumor experiment. TSP was administered i. p for 12d and the body weight, tumor, spleen and thymus weight were detected, meanwhile lymphocyte transformation, the activity of NK cells were determined with MTT assay. The immune organ weights and carbon clearance were examined in normal mice. RESULTS After treatment with TSP 25,50,100 mg · kg^-1 the inhibition rate （IR） on Hepatoma-22 and Sarcoma 180 were 17.8% ,27.6% ,34. 9% ; 32.8% ,36. 1% ,44.0% respectively. TSP 50,100 mg · kg^-1 also increased lymphocyte transformation, the activity of NK cells significantly in tumor-bearing mice （P 〈 0. 05 ）. The spleen and thymus weight and the phagocytosis of reticuloendothelial system in normal mice were enhanced obviously. CONCLUSION TSP has the antagonistic effect on transplanated tumor. It may be related with the enhancement of immunological function.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第2期93-95,共3页
Chinese Journal of Modern Applied Pharmacy
基金
国家九五科技攻关课题(96-901-06-68)
吉林省科技发展计划重点项目(20010443)
关键词
银耳孢糖
抗肿瘤
淋巴细胞转化
天然杀伤细胞
Tremella fuciformis spores Polysaccharides
anti-tumor
lymphocyte transformation
nature killer cells
