摘要
目的:探讨基质金属蛋白酶9(MMP-9)、金属蛋白酶特异性组织抑制因子1(TIMP-1)在子宫内膜异位症患者内膜标本中表达水平的变化及其临床意义。方法:收集子宫内膜异位症患者的异位内膜56例(观察组)和非内异症患者的子宫内膜标本18例(对照组),采用RT-PCR半定量方法检测内膜标本中MMP-9、TIMP-1 mRNA表达率及表达强度。结果:观察组和对照组所有内膜标本均有TIMP-1 mRNA表达;观察组和对照组标本中MMP-9 mRNA的表达率分别为58.9%和50%;观察组异位内膜中MMP-9 mRNA的表达强度高于对照组(P<0.05);TIMP-1 mRNA的表达强度低于对照组(P<0.05);对于MMP-9/TIMP-1比值,观察组明显高于对照组(P<0.01),早期(I-II期)明显高于III、IV期(P<0.01)。结论:子宫内膜异位症患者内膜中TIMP-1 mRNA的表达减弱,降低了对MMP-9的抑制作用;同时子宫内膜异位症患者内膜MMP-9 mRNA的表达增强,加强了其侵袭能力,易于发生种植转移,这些因素可能在子宫内膜异位症的发生发展过程中发挥重要作用。
Objective To investigate mRNA expression of matrix metalloproteinase 9（MMP-9） and tissue inhibitor of metalloproteinase 1 （TIMP-1）in ectopic endometriosis tissue and uterine endometrium from women without endometriosis. Methods Fifty-six women with endometriosis were selected as experiment group. Eighteen specimens of endometrium from reproductive women undergoing laparoscopic surgery without endometriosis were obtained as control group. The mRNA expressions of MMP-9 and TIMP-1 were detected with reverse transcription polymerase chain reaction. Results The expression of TIMP-1 mRNA was detected in all samples. The mRNA expression of MMP-9 of patients with endometriosis was higher than that in control group （P〈 0.05）. As for the mRNA expressions of TIMP-1, it was lower in patients with endometriosis than that in control group （P〈0.05）. The same result appeared in advanced stage compared with early stage. Conclusion It is suggested that MMP-9 and TIMP-1 may play an important role in the pathogenesis of endometriosis. Detection of MMP-9 and TIMP-1, especially the ratio of MMP-9/ TIMP-1 has an important clinical significance.
出处
《实用诊断与治疗杂志》
2007年第10期758-759,762,共3页
Journal of Practical Diagnosis and Therapy
