摘要
目的研究鼻咽癌(NPC)组织内EBV感染与c-myc、bcl-2、Bax的表达和癌细胞增殖、凋亡的相关性及与NPC预后的关系。方法原位杂交方法检测51例鼻咽非角化性癌(NKC)中EBERs的表达率,并用免疫组化EnVision二步法检测35例生存期<5年的NKC、60例生存期≥5年的NKC及30例鼻咽黏膜慢性炎症组织(NP)中EB病毒潜伏膜蛋白-1(LMP-1)、c-myc、bcl-2、Bax、Ki-67和M30的表达水平,并计数增殖指数(PI)和凋亡指数(AI)。结果①51例NKC患者EBERs的表达率为70.6%,LMP-1表达率为68.6%,两者呈正相关(P<0.005);②生存期<5年、≥5年及NP组中LMP-1、c-myc的阳性表达率依次递减,各组差异显著(P<0.05);③bcl-2和Bax在NKC组阳性率分别为66.3%和56.8%;bcl-2/Bax的比值与患者的预后相关;LMP-1的表达与bcl-2/Bax>1的表达呈正相关(P<0.05);④LMP-1、c-myc的表达及bcl-2/Bax的比值与PI指数呈明显正相关(P<0.05),而与AI指数无明显相关性;⑤LMP-1与c-myc的表达呈正相关(r=0.673,P<0.005),且二者共同表达时的PI值较单独表达时明显增高(P<0.05)。结论LMP-1、c-myc的表达及bcl-2/Bax的比值与NKC细胞的失控性增殖和预后密切相关,且LMP-1与c-myc在NKC发生、发展过程有相互增强的协同作用,提示上述基因可作为预测肿瘤恶性度及判定预后的参考指标。
Objective To investigate the relationship between the infection of Epstein-Barr virus （EBV） and the expression of c- myc, bcl-2 and Bax, PI, AI and prognosis in nasophyngearl carcinoma （NPC） . Methods We detected the positive expression of EBERs by in situ hybridization in 51 cases of NKC. An EnVision immunohistochemical method was used to detect the expression of LMP-1, c-myc, bcl-2, Bax, Ki-67, and M30 in 35 cases of nonkeratinizing carcinoma （NKC） with surviving less than 5 years, 60 cases of NKC with surviving over 5 years and 30 cases of non-ttunor nasopharyngeal tissue （NP）. The proliferating index and apoptotic index were counted by immunostaining with Ki67 and M30. Results （1） In 51 cases of NKC, the positive expression rate of EBERs was 70.59% （36/51）, and the positive expression rate of LMP- 1 was 68.63% （35/51） ; there was significant and positive correlation between the expression of LMP-1 and EBERs （r = 0.497, P 〈 0.005） . （2） The positive expression rates of LMP-1 and c-myc decreased successively in NKC with surviving less than 5 years group, surviving over 5 years group and NP group; the difference was significant （ P 〈 0.05） ; （3） The positive rates of bel-2 and Bax in NKC were 66.32% （63/95） and 56.84% （54/95）, respectively; and the ratio of bcl-2/Bax was positive correlated with prognosis; and the exprossion of LMP-1 was positive correlated with the bcl-2/Bax 〉 1 （ P 〈 0.05） ; （4） In 95 cases of NKC, the PI was highly related to the expression of LMP-1, c-myc and bcl-2/Bax （P 〈 0.05）; and there were not shown any significant difference between AI and the expression of antibodies mentioned above; （5） There was positive correlation between the expression of LMP-1 and c-myc （ r = 0.673, P 〈 0.005）, the PI in the co-expression of LMP-1 and c-myc was much higher than that in the single expression; the difference was significant （ P 〈 0.05）. Conclusions The expression of LMP-1, c-myc and index of bcl-2/Bax are highly related to the proliferation and prognosis of NKC. They might be reference indicators for evaluating the malignant grade and prognosis of NKC.
出处
《诊断病理学杂志》
CSCD
2007年第4期298-301,共4页
Chinese Journal of Diagnostic Pathology
基金
省长科研基金
贵州省科技厅基金资助(黔科合2004NYG044)
