摘要
目的研究正己烷代谢产物2,5-己二酮(2,5-HD)对雪旺细胞神经生长因子(NGF)及其p75神经营养素受体(p75NTR)表达水平的影响。方法取新生Wistar大鼠坐骨神经进行雪旺细胞原代培养,采用差速贴壁法和Thy-1.1抗体进行细胞纯化。采用免疫荧光法观察2,5-HD不同染毒浓度和染毒时间雪旺细胞NGF及其p75NTR表达水平的变化,图像分析软件Image-Pro Plus进行定量分析。结果2,5-HD 5～40 mmol·L^(-1)染毒24 h可以促进雪旺细胞NGF及其p75NTR的表达,呈浓度-效应关系;2,5-HD 10.0 mmol·L^(-1)染毒1～48 h,NGF及其p75NTR表达水平呈先增高后降低的趋势。结论2,5-HD可以促进雪旺细胞NGF及其p75NTR的表达,这可能是机体的一种自我保护机制,为使用NGF治疗正己烷中毒性周围神经病提供了依据。
AIM To study the effect of 2,5- hexanedione （2,5-HD）, a metabolite of n-hexane, on the expressions of nerve growth factor （ NGF ） and p75 neurotrophin receptor （ p75NTR ） in Schwann cells. METHODS Primary cultured Schwann cells were obtained from sciatic nerve of newborn rats and purified by method of adhering to plastic in different times and anti-Thy1. 1 antibody. The fluoroimmunoassay was used to detect the expressions of NGF and p75NTR and Image-Pro Plus was used to quantify the absorption of cells treated with 2,5-HD. RESULTS Compared with the con- trol group, the expressions of NGF and p75NTR in Schwann cells treated with 2,5-HD 5, 10, 20 and 40 mmol·L^-1 for 24 h increased in a concentration-effect manner. Treated with 2,5-HD 10 mmol·L^-1 for 1, 3, 6, 12, 24 and 48 h, the expressions of NGF and p75NTR in Schwann cells were up-regulated soon, and then down-regulated. CONCLUSION 2,5- HD increases the expressions of NGF and p75NTR in Schwann cells from sciatic nerve of rats, which may be a self-protection mechanism of body and provide scientific evidence for clinical therapy of chronic hexane poisoning with NGF.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2007年第4期271-277,共7页
Chinese Journal of Pharmacology and Toxicology
基金
国家重点基础研究发展规划(973)资助项目(2002CB512907)~~
