摘要
目的:观察大鼠在安静、急性力竭运动中、运动力竭即刻和恢复24h四种功能状态,肾组织总抗氧能力及丙二醛含量的变化。方法:实验于2005-10在曲阜师范大学体育科学学院生化实验室完成。①选用成年雄性Wistar大鼠32只。随机分为4组:安静对照组、游泳40min组、力竭即刻组和恢复24h组,每组8只。各组大鼠均进行2d适应性游泳。第3天,安静对照组大鼠不游泳直接取材;其余组大鼠尾部负重3%体质量的铅条游泳,不断用铁棒搅动迫使大鼠游动。游泳40min组、力竭即刻组和恢复24h组大鼠在游泳40min后、力竭运动后即刻、力竭运动后24h取材。力竭标准:大鼠沉入水面下10s,托起后再次沉下,反复两三次,视为力竭。②按南京建成生物工程研究所试剂盒说明进行操作测定肾组织总抗氧能力、丙二醛含量。③计量资料差异比较采用t检验。结果:纳入大鼠32只,均进入结果分析,无脱失值。①肾组织总抗氧能力:游泳40min组、力竭即刻组分别为(3.63±0.64),(3.65±0.99)kU/g,高于安静对照组[(2.99±0.17)kU/g,P<0.05);力竭即刻组略高于游泳40min组,但差异不明显(P>0.05);恢复24h组为(2.96±0.67)kU/g,略低于安静对照组,差异也不明显(P>0.05)。②肾组织丙二醛含量:游泳40min组、力竭即刻组分别为(4.38±1.34),(4.74±1.65)μmol/g,高于安静对照组[(3.45±0.28)μmol/g,P<0.05,0.01];力竭即刻组高于游泳40min组,但差异不明显(P>0.05)。恢复24h状态,达到最大值为(7.66±1.52)μmol/g,高于其他3组(P<0.01)。结论:急性力竭运动大鼠肾组织内抗氧化能力和丙二醛含量较安静状态时提高,力竭运动后24h总抗氧化能力恢复至安静状态水平,丙二醛含量仍高。
AIM： To observe the functional status of rats under 4 different conditions： at rest, acute exhaustive exercising, exhaustive instant in exercise and 24 hours after convalescence as wall as the changes in total antioxidant ability and malondialdehyde （MDA） in renal tissues. METHODS： The experiment was conducted in the Biochemical Laboratory of College of Physical Education, Qufu University in October 2005. （1） Thirty-two adult male Wistar rats ware selected and randomly divided into 4 groups; Quiet control group, 40-minute swimming group, exhaustive instant group and 24-hour convalescence group, and there ware 8 rats in each group. Rats in each group performed adaptive swimming for 2 days. Materials ware drawn from the quiet control group without swimming. Rats in other groups ware required to swim with tail loading of lead bar, which was as heavy as 3% of the body mass by continuously stirring the water with iron bar. Rats in the 40-minute swimming group, exhaustive instant group and 24-hour convalescence group ware executed respectively at 40 minutes after swimming, at the instant of exhaustive swimming and 24 hours after the exhaustive swimming. Standards for exhaust： Rats sank into the water for 10 s and sank after being pull out, which was repeated twice or three times. （2） Total antioxidant ability and MDA content of renal tissues ware determined according to the instruction on the kit of Nanjing Jiancheng Institute of Biotechnology. （3） Differences in measurement data ware compared with ttest. RESULTS： A total of 32 enrolled rats were involved in the analysis of results, and no one withdrew from the study. （1） The total antioxidant ability of renal tissues： It was （3.63±0.64） kU/g and （3.6±0.99） kU/g respectively in the 40-minute swimming group and exhaustive instant group, which ware higher than that in the quiet control group [（2.99±0.17） kU/g,P 〈 0.05], and it was higher in the exhaustive instant group than that in the 40-minute swimming group, whereas the differences were not significant （P〈 0.05）. It was （2.96±0.67） kU/g in the 24-hour convalescence group, which was a little lower than that in the quiet control group, and the differences ware not remarkable （P〉 0.05）. （2） MDA in the renal tissues; It was （4.38±1.34） μmol/g and （4.74±1.65） μmol/g respectively in the 40-minute swimming group and the exhaustive instant group, which were higher than that in the quiet control group [（3.45±0.28） μmol/g,P 〈 0.05,0.01]. It was higher in the exhaustive instant group than that in the 40-minute swimming group, while the differences ware not marked （P〉 0.05）. It peaked at 24 hours after convalescence; （7.66±1.52） μmol/g, which was higher than 3 other groups （P 〈 0.01）. CONCLUSION： The total antioxidant ability and level of MDA in the renal tissues of rats in acute exhaustive swimming are higher than those in rats under quiet condition. Twenty-four hours after exhaustive swimming, the total antioxidant ability recovers to that under quiet condition, while MDA is Still higher.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第30期6016-6018,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
中国矿业大学科研基金项目(0ER4568)~~
