摘要
鸡毒霉形体（ＭＧ）中毒株Ｓ６在固体平板上３次克隆后，在无细胞培养基上传１００代以上进行致弱培养。用ＭＧＳ６Ｆ１０８株培养物在雏鸡和ＳＰＦ鸡胚上作６次回归感染，证明ＭＧＳ６Ｆ１０８株不返强。通过气管环培养、纤毛运动损伤试验及对鸡点眼、滴鼻、左胸气囊注射不同代次培养物（Ｆ１０８、Ｆ１１３、Ｆ１２１、Ｆ１５０、Ｆ１５３），检查气囊病变和病原体再分离，结果证明Ｆ１０８以上代次的菌株低毒、安全。
After 3 sequential cloning in PPLO agar plate, laboratory attenuated strain F 108 of M. gallisepticum , derived from strain S 6 was selected. By more than 100 in vitro passages, cloned strain F 108 did not revert to the former level of pathogenicity in 6 back passages in chickens. With the observations of pathogenic effects on chick tracheal ring culture, and with aerosol challenge and ocular instillation, F 108 F 113 F 121 F 150 F 153 could induce significant serological response and were weakly virulent.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第2期90-94,共5页
Journal of Nanjing Agricultural University
关键词
鸡毒霉形体
菌株
气管环培养
克隆
致弱
Mycoplasma gallisepticum
strain
tracheal ring culture
back passage
clone
attenuation
