摘要
本文选择人结肠癌相关抗原Ｓｃ３Ａ抗原低表达株ＳＷ１１１６，用不同剂量的ｒＨｕＩＦＮ－γ体外刺激２４～７２ｈ，计算Ｓｃ３Ａ阳性细胞的百分率。经间接免疫荧光染色、流式细胞仪分析，结果表明刺激２４ｈＰＢＳ对照组ＳＷ１１１６Ｓｃ３Ａ阳性率为１１．３％，２０ｕ／ｍｌｒＨｕＩＦＮ－γ组Ｓｃ３Ａ阳性率为９５．５％。免疫细胞化学染色显示，对照组阳性细胞是散在淡染，ｒＨｕＩＦＮ－γ刺激组细胞浓染成团。荷人结肠癌裸鼠腹腔注入ＣＨＣＩＦＮ－γ和１３１Ｉ－ＭｃＡｂ（抗Ｓｃ３Ａ）标记物，分别于第２、４、６和８ｄ进行γ射线照相、生物分布和肿瘤摄取率测定。结果显示，ｒＨｕＩＦＮ－γ＋１３１Ｉ－Ｓｃ３Ａ组瘤／血（Ｔ／Ｂ）比值和肿瘤／非肿瘤（Ｔ／ＮＴ）之比均最高，分别为３．６和６．５７。肿瘤放射性摄取率仍以ｒＨｕＩＦＮ－γ＋１３１Ｉ－Ｓｃ３Ａ和１３１Ｉ－Ｓｃ３ＡＦ（ａｂ’）２两组为高，分别是２２．３％和２２．６％。显像结果与生物分布相一致。Ｓｃ３ＡＦ（ａｂ’）。无论有无ｒＨｕＩＦＮ－γ均在第４ｄ显像清晰，而完全ＭｃＡｂ组无论有无ｒＨｕＩＦＮ－γ均在第６ｄ显像清晰。但ｒＨｕＩＦＮ－γ＋１３１Ｉ－Ｓｃ３ＡＦ（ａｂ′）２和ｒＨｕＩＦＮ－γ＋１３１?
Human colorectal carcimoma cell line SW1116 with low expression of tumor associated antigen Sc3A was stimulated by rHuIFN-γin vitro for 24 h,then stained and analyzed by FACS.The result showed that the positive rate of Sc3A rose from 11. 3% in PBS control group to 95. 5% inrHuIFN-y stimulated group. In immuno-histochemistry analysis, the Positive cell in control group waslightly stained in a scattered pattern, while in the rHuIFN-γ stimulsting group densely stained in asmeared pattern.The mude mice planted with human colorectal carcimoma was injected intraperitoneally withrHuIFN-γ and 131 I-McAb to Sc3A. Radiophotographs was taken, and radiodistribution and radiouptake checked at 2, 4, 6 and 8 days after injection. The tumor/bltal(T/B) ratio and tumor/nontumor(T/NT) ratio of radioactivity in rHuIFN-γ+ 131I-Sc3AMcAb gtoup was 3. 6 and 6. 57 respectively, much higher than that of control group. The radio uptake rate in the treatment group, being22.3% and 22. 6% when intact McAb to Sc3A or its F(ab' )2 fragment was used respectively,was also higher than that of control group. Result of radiograph was in accordance with the result of radiodistribution. Although in the absence of rHuIFN-γ we could get clear image in 4 or 6 days for F(ab')2 orintact antibody group respectively, in 131I-Sc3A McAb plus rHuIFN-γ treatment group the image wasmore clear.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1996年第1期10-15,共6页
Chinese Journal of Cellular and Molecular Immunology
