摘要
目的:通过测定慢性心理应激和力竭运动后大鼠血清中血尿素氮的含量及全血细胞的数量,揭示运动及心理应激对免疫功能的影响。方法:实验于2004-09/10在江西师范大学体育学院实验室完成。健康雄性SD大鼠32只,随机分为4组:对照组,心理应激组,力竭运动组,力竭运动加心理应激组,每组8只。心理应激方法为开灯-电击10s-电击后3s关灯。间隔10s后重复1次,共3次,以上为1个轮次的应激刺激,每天3个轮次,在10min内完成,心理应激时间为2周。力竭运动方法以35m/min的速度,跑台坡度为15°运动至力竭。各训练组动物安排在最后一次训练后24h与对照组大鼠同步处死,测定大鼠血清中血尿素氮含量及全血细胞的数量。结果:进入结果分析为30只,力竭运动加心理应激组有2只大鼠在力竭运动后死亡。①力竭运动组血尿素氮明显升高,与对照组比较,差异有显著性[(10.08±1.44),(7.18±1.51)mmol/L,F=2.90,P=0.047];心理应激组和力竭运动加心理应激组也有升高的趋势,但差异没有显著性(F=2.55,P=0.09)。②与对照组比较,力竭运动组白细胞数略高,力竭运动加心理应激组单核细胞百分比、中性粒细胞数、中性粒细胞百分比有升高趋势,差异没有显著性(P>0.05)。结论:力竭运动使血尿素氮升高,力竭运动及心理应激使血清中白细胞数略高,单核细胞百分比、中性粒细胞数、中性粒细胞百分比有升高趋势,说明运动及心理应激条件下,机体调动了非特异性免疫的防御机制。
AIM： To reveal the effect of exercise and psychological stress on immune function by determining the content of blood urea nitrogen and the number of complete blood cells in rats with chronic psychological stress after exhaustive exercise. METHODS： The experiment was conducted in the Laboratory of Physical Education Department of Jiangxi Normal University between September and October 2004. Thirty-two SD adult rats were randomly divided into four groups： control group, psychological stress group, exhaustive exercise group, exhaustive exercise and psychological stress group with eight rats in each group. The procedures of the psychological stress were： First, turn on the light. Second, 10-s shock. Third, tum off the light at 3 s after electric shock. The psychological stress was given for three times with a 10 s interval between each two. All of abeve-mentioned procedures were taken as a cycle of stress and there were totally 3 cycles conducted each day, which were accomplished within 10 minutes. The psychoiogical stress was performed for 2 weeks. The exhaustive exercise was conducted at the rate of 35 m/minute on run platform with the slope of 15°. All rats were executed at 24 hours after the last training, and the content of blood urea nitrogen as well as the number of complete blood cells were determined in rats. BESULTS： A total of 30 rats were involved in the analysis of results, and two rats in the exhaustive exercise and psychological stress group died after exhaustive exercise. ① The content of blood urea nitrogen in the exhaustive exercise group was significantly increased than that in the control group [（10.08±1.44） mmol/L, （7.18±1.51） mmol/L, F=2.90,P =0.047], and that in the psychological stress group as well asthe exhaustive exercise group and psychological stress group were increased, while the differences were not remarkable （F=2.55, P =0.09）. ②Compered with the control group, the number of white blood corpuscle （WBC） in the exhaustive exercise group was a little less. There was an increasing trend in the percent of monocyte, number of neutrophilic granulocyte and the percent of neutrophilic granuiocyte in the exhaustive exercise and psychological stress group, while the differences were not significant （P〉 0.05）. CONCLUSION： Exhaustive exercise can increase the content of blood urea nitrogen, and exhaustive exercise integrated with psychological stress can increase the WBC number, percents of monocytes, number of neutrophilic granulocyte and the percent of neutrophilic granuiocyte, which indicate that the non-specificity defense mechanism is activated under exercise and psychological, stress.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第17期3361-3363,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
江西师范大学博士基金资助项目(1052)~~
