摘要
以水稻土浸提液为接种液、人工合成的无定形氧化铁为唯一的电子受体、添加的苯系物(甲苯、苯胺、苯酚、苯甲酸)为唯一碳源,在适量无机N,P,K营养供给条件下,采用厌氧恒温混合培养方法,研究了厌氧微生物对氧化铁还原的影响。结果表明,由水稻土中提取的微生物可以利用苯系物作为唯一碳源,在厌氧降解的同时将电子转移给F e(Ⅲ),使其还原为F e(Ⅱ);利用不同苯系物的铁还原过程难易程度有所差异,铁还原率表现为苯甲酸最高(53.99%),甲苯最低(19.55%),利用苯系物的铁还原率均低于以葡萄糖为碳源时的铁还原率(71.16%);不同浓度苯系物对氧化铁还原过程有不同程度的影响,在一定浓度范围内,随着苯系物浓度的增加其对氧化铁还原过程具有促进作用,而高浓度苯系物则对氧化铁还原过程表现出抑制趋势;以2.0 mm o l/L苯系物作为碳源时,蒽醌-2,6二-磺酸盐(AQDS)可明显增加F e(Ⅱ)的累积量。
The paddy soil extraction was incubated anaerobically as inoculum and dissimilatory iron re duction was investigated during anaerobic co culture containing ferrihydrite as sole electron aeceptor,ben zene analogs （toluene ,aniline, phenol, benzoic acid） as sole carbon source, under suitable supplies of inorganic N,P and K nutrition. The results indicated that,the microbe in the paddy extraction could transfer electrons to Fe（ Ⅲ ）,utilizing benzene analogs as sole carbon source when microbe was degraded;the iron reduction rates were different ,the maximal reduction was 53.99% with benzoic acid as carbon source and the minimum reduction was 19. 55% with toluene as carbon source; the reduction level with benzene analogs as carbon source was less than that with glucose as carbon source （71.16%）;different concentrations of benzene analogs had different effects on iron reduction;under suitable concentration range of benzene analogs,dissimilatory iron reduction depending upon the microorganism activities could be strengthened ,however,there was a trend that iron reduction was to be suppressed when the concentration of benzene analogs was too high. The addition of anthraquione-2,6-disulfonate during co culture could obviously increase Fe （Ⅱ ） level with 2. 0 retool/L of benzene analogs as carbon source. This showed that anthraquione-2,6-disulfonate could promote the transference of electrons from the benzene analogs to ferrihydrite.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2006年第10期101-106,112,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(40271067)
教育部博士学科点专项科研基金项目(20020712009)
关键词
异化铁还原
苯系物
水稻土
蒽醌-2
6-二磺酸盐
dissimilatory iron reduction
benzene analogs
paddy soil
anthraquione-2,6-disulfonate
