摘要
目的：建立高效液相测定膨化食品中的诱惑红的含量。方法：EclipseXDB—C18柱分离，以甲醇-0．02mol／l乙酸铵（40：60）为流动相，流速为1．0mL· min^-1，检测波长为540nm，柱温25℃，进样体积为50μl。结果：线性范围为2．306～36．896μg/mL，相关系数为0.9992，平均回收率范围为85．5％-87．7％；精密度为1．17％（n=6）。结论：该法精密度、准确度均能满足检测方法的要求。
Objective：To develop a HPLC method for the determination of fancy red in puffing food. Methods：The determination was performed on a Eclipse XDB -C18 column（4.6 ×250 mm,5 μm）with the mobile phase consisting of methanol -0.02 mol· L^-1 ammonium acetate （40：60） at 25℃ ,The flow rate was 1.0 mL· min^-1. The detection was made at 540nm . The injection volume was 50μl. Results： The method offered good linearity from 2. 306 - 36, 896 μgmL· L^-1, relativity coefficient was 0. 9992 . The average recovery was from 85.5% to 87.7% . The precision of method was 1.17% （ n = 6 ）. Conclusions ： The developed HPLC method was accurate and precision, and could be used for the determination of fancy red in puffing food.
出处
《中国民康医学》
2006年第19期837-838,共2页
Medical Journal of Chinese People’s Health
