摘要
To investigate the low temperature on germination capacity （LTG） a double haploid rice （DH） population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica line AAV002863 was used to construct a linkage map with 140 SSR markers, The germination rate in Zhenshan 97B and AAV002863 was 79,7% and 30,1%, while in DH population it ranged from 0 to 100% at 15℃ after 6 days, Quantitative trait loci （QTLs） controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10 was 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increased the LTG at qLTG-3 region, while allele from AAV002863 increased the LTG at qLTG-IO region. One pair of epistatic interaction was detected between loci on chromosomes 3 and 10. The main-effect of QTL on chromosome 10 was also involved in epistatic interaction.
To investigate the low temperature on germination capacity （LTG） a double haploid rice （DH） population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica line AAV002863 was used to construct a linkage map with 140 SSR markers, The germination rate in Zhenshan 97B and AAV002863 was 79,7% and 30,1%, while in DH population it ranged from 0 to 100% at 15℃ after 6 days, Quantitative trait loci （QTLs） controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10 was 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increased the LTG at qLTG-3 region, while allele from AAV002863 increased the LTG at qLTG-IO region. One pair of epistatic interaction was detected between loci on chromosomes 3 and 10. The main-effect of QTL on chromosome 10 was also involved in epistatic interaction.
