摘要
目的:探讨红花注射液对大鼠肾缺血再灌注损伤凋亡相关基因Caspase-3和bcl-2表达的影响。方法:将75只SD大鼠随机分为正常对照组、缺血再灌注组和治疗组。建立肾缺血再灌注损伤模型,检测肌酐和血尿渗透压值评价肾功能变化,TUNEL法检测凋亡率,免疫组化检测Caspase-3和bcl-2蛋白表达。结果:与正常对照组相比,缺血再灌注组肾功能明显减退,凋亡率显著增加、Caspase-3和bcl-2表达明显增强(P<0.01);运用红花注射液治疗后,与缺血再灌注组相比,治疗组肾功能明显改善,凋亡率显著减少、Caspase-3的表达明显减弱而bcl-2表达明显增强(P<0.01)。结论:在肾缺血再灌注损伤过程中,红花注射液具有抑制Caspase-3表达和促进bcl-2表达的作用。
Objective： To investigate the effect of carthamus tinctorius injection on expression of caspase-3 and bcl-2 after renal ischemia/reperfusion injury. Methods： Seventy-five SD rats were randomly divided into three groups （n=25, in each）：normal control group, ischemia/reperfusion group and treatment group. After the renal ischemia/reperfusion injury model was estahlished, the serum creatinine . the urine osmotic pressure and the blood osmotic pressure were measured to evaluate the change of renal function; the apoptosis rate in ischemia/reperfusion renal tissure were measured by TUNEL method; the caspase-3 and bcl-2 concentration were measured by immuno-histochemistry. Results ：The renal function got worse,and the apoptosis rate, bcl-2 and caspase-3 concentration were significantly higher in ischemia/reperfusion group than those in normal control group .As compared with ischemia/reperfusion group, the renal function was improved, the apoptosis rate decreased, bcl-2 expression increased and caspase-3 expression decreased significantly in treatment group. Conclusion：Carthamus tinctorius injection has significant effect on the up-regulation of bcl-2 and down-regulation of caspase-3 expression in renal ischemia/reperfusion injury.
出处
《重庆医科大学学报》
CAS
CSCD
2006年第4期532-536,共5页
Journal of Chongqing Medical University
