摘要
目的探讨督脉电针对脊髓全横断损伤处移植的神经干细胞存活、分化和迁移的影响。方法将20只成年SD大鼠分为神经干细胞移植14d组(NSCs 14d组)、督脉电针+神经干细胞移植14d组(电针NSCs 14d组)、神经干细胞移植30d组(NSCs 30d组)和督脉电针+神经干细胞移植30d组(电针NSCs 30d组)4组。所有动物均实施T10段脊髓全横断手术,其中电针组和电针NSCs组于术后5d进行电针治疗。分别于术后14d和30d取材检测移植在脊髓损伤处的神经干细胞存活、分化和迁移情况。结果1.电针NSCs 14d组或电针NSCs 30d组移植的神经干细胞存活数量均多于NSCs 14d组或NSCs 30d组,但是电针NSCs 30d组或NSCs 30d组移植的神经干细胞存活数量均少于电针NSCs 14d组或NSCs 14d组。2.电针NSCs 30d组和NSCs 30d组的脊髓全横断损伤处及其相邻的组织均有少量移植的神经干细胞呈现微管相关蛋白2(MAP2)阳性染色。3.电针NSCs 30d组和NSCs 30d组的脊髓全横断损伤处及其相邻的组织均可观察到较多移植的神经干细胞呈现胶质原纤维酸性蛋白(GFAP)阳性染色。4.在电针NSCs 14d组或电针NSCs 30d组,移植的神经干细胞向脊髓损伤处尾端组织迁移的距离明显长于NSCs 14d组或NSCs 30d组。结论督脉电针能够促进大鼠脊髓全横断损伤处移植的神经干细胞存活,这些细胞能分化为MAP2或GFAP阳性细胞;督脉电针对移植在脊髓损伤处的神经干细胞向宿主脊髓组织迁移方向有一定的影响。
Objective To explore the effects of DU meridian electroacupuncture on the survival, differentiation and immigration of neural stem cells （NSCs） transplanted in the injured site of rat spinal cord transection. Methods Twenty adult SD rats were divided into NSCs transplanted 14d group （NSCs14d group）, DU meridian electroacupuncture plus NSCs transplanted 14d group （EA ＋ NSCs14d group）, NSCs transplanted 30d group （NSCs30d group） and DU meridian electroacupuncture plus NSCs transplanted 30d group （EA ＋ NSCs30d group）. T10 spinal cord segments of all animals were completely transected. The rats of EA ＋ NSCs14d group and EA ＋ NSCs30d group were treated with DU electroacupuncture at 5 days after operation. The sacrifice times of the rats were respectively at 14 days and 30 days after operation. The spinal cords were taken for observing the survival, differentiation and immigration of neural stem cells transplanted in the injured site of rat spinal cord transection. Results 1 .The surviving number of transplanted NSCs in EA ＋ NSCs14d group or EA ＋ NSCs30d group was more than that of NSCs14d group or NSCs30d group, and the surviving number of transplanted NSCs in EA ＋ NSCs30d group and NSCs30d group was less than that in EA＋ NSCs14d group and NSCs14d group. 2.Some transplanted NSCs at injured site of transected completely spinal cord and neighboring tissue showed microtubule association protein 2 （MAP2） positive staining in EA ＋ NSCs30d group or NSCs30d group 3. Many transplanted NSCs at injured site of transected completely spinal cord and neighboring tissue could be observed to show glial fibrillary acidic protein （GFAP） positive staining in EA ＋ NSCs 30d group or NSCs 30d group. 4. The immigrating distance of transplanted NSCs toward caudal tissue of injured site of spinal cord was longer in EA ＋ NSCs 14d group and EA ＋ NSCs 30d group than that in NSCs 14d group and NSCs 30d group, Conclusion DU meridian electroacupuncture may promote the survival of neural stem ceils transplanted in injured site of rat spinal cord transeeted completely, and these cells can differentiate into MAP2 or GFAP possive cells. DU meridian electoacupuncture may affect the immigrating direction of neural stem cells transplanted at injured site of spinal cord toward host spinal cord tissue.
出处
《解剖学报》
CAS
CSCD
北大核心
2006年第4期381-386,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30472132)
广东省中医药管理局科研基金资助项目(101139和303013)
关键词
督脉电针
脊髓损伤
移植细胞存活
移植细胞迁移
神经干细胞移植
大鼠
DU meridian electroacupuncture
Spinal cord injury
Cell survival
Cell immigration
Neural stem cells transplanted
Rat
