摘要
目的:建立一种简便、有效、稳定的乙型肝炎病毒(HBV)感染的动物模型,观察该模型动物体内不同时间点各种HBV标志物的表达情况．方法:以流体动力学法尾静脉注射BALB／c 小鼠pcDNA3．1-HBV,1 wk内检测各种HBV 标志物,时间分辨免疫荧光分析法(IFMA)检测血清中HBsAg,HBeAg,抗HBs,抗HBe,抗 HBc,荧光定量PCR法(FQ-PCR)检测血清HBV DNA,免疫组织化学法检测肝组织HBsAg和 HBcAg．结果:成功建立一种急性HBV感染动物模型, 第1天血清中HBsAg表达达高峰,后逐渐降低, HBeAg表达量少,分别在第4、5、7天检测到抗HBc,抗HBe,抗HBs,d1 HBV DNA滴度亦达高峰,后渐下降,免疫组织化学示HBsAg呈胞质内弥漫性分布,HBcAg亦主要为胞质型分布．结论:以流体动力学法建立的HBV模型是一种新型有效的HBV感染动物模型,能稳定较高水平表达大部分HBV标志物．
AIM： To develop a simple, convenient, efficient and stable animal model of hepatitis B virus （HBV） infection, and observe the dynamic changes of viral replication as well as expression in the model.
METHODS： pcDNA3.1-HBV was delivered into BALB/c mice using the hydrodynamic tail vein injection method. In a week, the levels of HBsAg, HBeAg, anti-HBs, anti-HBe, and anti-HBc were determined with time-resolved immunofluorometric assay （IFMA） kit, and the titers of HBV DNA were analyzed by fluorogenic quantitative polymerase chain reaction （FQ-PCR）. In addition, viral specific proteins （HBsAg and HBcAg） in the liver were assayed by immunohistochemical staining.
RESULTS： A mouse model of acute HBV infection was developed successfully. HBsAg expression reached the peak on day 1, and dropped gradually. The level of HBeAg was low, and the level of anti-HBc, anti-HBe, and anti-HBs were first detected on day 4, 5 and 7, respectively. The titers of HBV DNA also peaked on day 1 and declined thereafter. HBsAg was positively scattered in the cytoplasm of hepatocytes, and HBcAg was mainly positively expressed in the cytoplasm of hepatocytes, too.
CONCLUSION： This is a novel and effective animal model of HBV infection, in which HBV can replicate and express stably.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第11期1052-1057,共6页
World Chinese Journal of Digestology
基金
湖南省科技厅科技计划重点资助项目
No
05SK2001
