摘要
采用小鼠胎肝细胞CFU-E测定技术,以及小鼠骨髓细胞体外CFU-E和BFU-E培养技术。观察钝顶螺旋藻藻蓝蛋白对小鼠红系祖细胞生成的影响。贫血小鼠模型建立采用60Coγ-射线辐照并注射盐酸苯肼。正常小鼠皮下注射藻蓝蛋白(C-PC)(5mg.kg-1)5d后,能明显增加CFU-E和BFU-E的集落数。贫血小鼠皮下注射藻蓝蛋白5d后,C-PC能明显增加白细胞、红细胞和血红蛋白的数量。以C-PC代替培养体系中的促红细胞生成素(EPO)或爆式集落刺激因子(BPA)来培养CFU-E和BFU-E,结果表明C-PC具有较高的EPO样活性,12.5ng的C-PC相当于1.06 U的EPO,其比活性约为84,800U.mg-1C-PC。实验还显示C-PC有较弱的BPA活性。
To investigate the effects of C-phycocyanin （C-PC） from Spirulina platensis on erythropoiesis in mice. The colony forming unit-erythroid （CFU-E） and burst forming uniterythroid （BFU-E） were determined using micro-methycellulose culture method in vitro. The erythropoietin （EPO）-like activity of C-PC was examined using the technique of CFU-E culture of fetal liver cells in mice in vitro. Anemic mice models were established by ^40Co γ- ray irradiation （SGy） and intra-peritoneal injected with benzohyarazine hydrochloride. After normal mice were intra-peritoneal injected with C-PC （50 mg · kg^-1） for 5d, C-PC provided the only increase in the numbers of CFU-E and BFU-E-derived colonies. C-PC exhibited higher EPO-like activity. 12.5 ng of C-PC could match with EPO 1.06U. The specific activity of C-PC was 84,800 U · mg^-1 C-PC. When anemic mice were intra-peritoneal iniected with C-PC for 5d, on dlO the leukocyte, erythrocyte and hemoglobin were significant increased
出处
《中国海洋药物》
CAS
CSCD
2006年第2期32-36,共5页
Chinese Journal of Marine Drugs
