摘要
目的:探讨具有益气固本、化瘀泄浊作用的中药复方制剂肾衰宁灌肠液(简称肾衰宁,SSN)防治慢性肾功能衰竭(CRF)的作用机制。方法:采用双抗夹心ELISA法,观察体外培养的人肾小球系膜细胞分别加入重组rhIL-1β(A组)及rhIL-1β+SSN大鼠血清(B组)后产生细胞间黏附分子1(ICAM-1)和血管细胞黏附分子1(VCAM-1)的水平。结果:A组人肾小球系膜细胞产生ICAM-1和VCAM-1增加,B组低于A组,且随SSN药物浓度的增加ICAM-1和VCAM-1越少。结论:SSN对rhIL-1β刺激后肾小球系膜细胞所产生的ICAM-1和VCAM-1有明显的抑制作用,提示SSN抑制系膜细胞自分泌ICAM-1和VCAM-1是预防肾小球硬化发生发展的重要作用机制之一。
Objective： To study the mechanism of the traditional Chinese drug-shenshuaining （SSN） which can discharge the turbid, tonfing Qi and activating blood, and to explore its therapeutic effects on chronic renal failure （CRF）. Methods： huMsC cultured in vitro were added in rhIL-1β （group A） and rhIL-1β＋SSN （group B） respectively, and the levels of ICAM-1 and VCAM-1 were observed by ELISA. Results： ICAM-1 and VCAM-1 levels increased in group A. ICAM-1 and VCAM-1 in group B were lower than those in group A, and decreased with SSN dosage increasing. Conclusion： ICAM-1 and VCAM-1 produced by huMsC can be inhibited by SSN. It is one of the important mechanisms of preventing the occurrence and development of glomerulosclerosis.
出处
《天津医药》
CAS
北大核心
2006年第3期183-184,共2页
Tianjin Medical Journal
关键词
肾衰宁
灌肠
肾小球膜
肾功能衰竭
慢性
胞间粘附分子1
血管细胞粘附分子-1
shenshuaining enema glomerular mesangium kidney failure,chronic intercellular adhension molecule-1 vascular cell adhension molecule-1
