摘要
建立了固相萃取净化和高效液相色谱法测定板鸭中几种肌间磷脂的方法。板鸭肌肉中的磷脂用氨丙基硅胶固相萃取柱纯化，然后用高效液相色谱正相柱分离。通过紫外检测器和蒸发光散射检测器串联检测显示，该测定方法回收率为81.0％～99．0％，相对标准偏差为1．5％-2．3％。将该法应用于板鸭样品的分析获得了较好的效果，经测定，板鸭肌间脑磷脂、卵磷脂、磷脂酰丝氨酸、神经鞘磷脂和溶血磷脂酰胆碱分别占肌间脂肪的8．79％、14．67％、0．11％、0．52％和0．27％。
A method of solid phase extraction （SPE） and high performance liquid chromatography （HPLC） for the determination of phospholipids in salted duck muscle was established. The phospholipids from salted duck muscle were depurated by LRC-NH2 SPE. Then the phospholipids were separated through NP-HPLC, and detected with the UV detector and ELSD, which were installed in series. The recovery was 81.0%-99. 0%, and relative standard deviations were 1.5%-2.3%. This method was successfully used in the analysis of salted duck sample, the proportion of phosphatidylethanolamine （PE）, phosphatidylcholine （ PC）, phosphatidylserine （ PS ）, sphingomyelin （ SPH ） and lysophosphatidylcholine （ LPC ） in muscle fat were 8. 79%, 14. 67%, 0. 11%, 0. 52% and 0. 27% respectively.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2006年第1期108-111,共4页
Journal of Nanjing Agricultural University
基金
国家863计划资助项目(2002AA248031)
国家"十五"肉品专项资助项目(2001BA501A24)
关键词
固相萃取
磷脂
板鸭
肌间
高效液相色谱
solid phase extraction （SPE）
phospholipids
salted duck
muscle
HPLC
