摘要
目的探讨大鼠眼钝挫伤后晶状体上皮细胞HSP70和HSP27的表达，并给予预先的热刺激（热休克），观察热休克对晶状体上皮细胞HSP70和HSP27表达的调节。方法48只Sprague-Dawley（SD）大鼠，应用随机数字表法随机分成扑打组和水浴组，每组24只鼠，右眼为实验眼。扑打组：20g铁球20cm高度拍打眼球100次。水浴组：给大鼠体温提高至40．5～41．5℃8min诱导出热耐受，2～3h后再给予20g铁球20cm高度拍打眼球100次。逆转录聚合酶链反应检测晶状体上皮细胞HSP70和HSP27基因的表达丰度。结果钝挫性眼外伤可造成品状体上皮细胞HSP70基因表达的增强，扑打眼球后1hHSP70表达升高至4．59±0．12，3h后达到高峰7．72±0．27，24h后降至正常1．32±0．14。预先的热刺激导致HSP70基因表达增高至1．83±0．30。两组HSP27基因表达丰度均无明显改变。结论钝挫性眼外伤中晶状体上皮细胞HSP70表达的增强提示HSP70可能在钝挫性外伤过程中对晶状体变性蛋白起保护作用，给予预先的热刺激可能通过提高HSP70的表达保护晶状体。
Objective To study the dynamic expression of heat shock protein （HSP） 70 and HSP27 in lens epithelial ceils （LECs） in contused eyes of rat model and to detect the dynamic change of the expression of HSP70 and HSP27 after thermotolerance. Methods Forty-eight Sprague-Dawley （SD） rats were randomized into two groups： contusion group and thermotolerance group. Contusion eyes were induced by dropping a steel ball of 20 g from the height of 20 cm for 100 times. Thermotolerance was induced by raising the core body temperature of rat to 40. 5-41.5 ℃ for 8 min, 2-3 h before eyes contusion. HSP70 and HSP27 expression in the LEC were measured using one-tube RT-PCIL Results Contusion of eyes resulted in increase of HSP70 expression. Expression of HSP70 increased to 4. 59 ± 0. 12 at 1 h after contusion, reached the highest level of 7.72 ±0. 27 at 3 h after contusion, and reduced to the normal level of 1.32 ± 0. 14 at 24 h after contusion. Preconditioning hyperthermia resulted in significantly increase of HSP70 expression. There was no significant change of HSP27 expression in beth groups. Conclusions Increased expression of HSP70 in LECs of contused eyes possibly protects the proteins of lens against degeneration. Thermal preconditioning might suppress lens injury induced by contusion via increasing the expression of HSP70.
出处
《中华眼科杂志》
CAS
CSCD
北大核心
2006年第3期241-245,共5页
Chinese Journal of Ophthalmology
基金
福建省科技厅青年科技人才创新项目(2004J073)
教育部高等学校博士学科点专项科研基金资助项目(301066)
关键词
热休克蛋白质类
晶体
上皮细胞
眼损伤
白内障
逆转录聚合酶链反应
Heat-shock proteins
Lens, crystalline
Epithelial ceils
Eye injuries
Cataract
Reverse transcriptase polymerase chain reaction
