摘要
目的探讨妊娠肝内胆汁淤积(ICP)鼠心肌酶谱和胎鼠心肌细胞超微结构的变化及其发病机制。方法取孕14 d大鼠40只,随机分为4组,每组10只。3个实验组分别注射剂量5．0、2．5、1．2mg/(kg·d)的苯甲酸雌二醇(EB),以诱导孕鼠产生不同程度的肝内胆汁淤积,测定孕晚期血清心肌酶谱的变化,用电镜观察胎鼠心肌超微结构的变化。对照组注射同体积的生理盐水。结果实验组胎鼠心肌细胞核内染色质分布异常,胞质稀疏,线粒体肿胀、嵴断裂或消失,出现膜样退变,闰盘间隙增大。各实验组总胆酸与注射EB前和对照组相比有所升高,高、中剂量组差异有显著性(P<0．01,P<0．05);与对照组比较,谷草转氨酶明显升高(均P<0．05);乳酸脱氢酶也不同程度升高,高、中剂量组差异均有显著性(P<0．05);高剂量组的肌酸激酶同工酶显著升高(P<0．05)。结论 EB诱导的ICP可以引起孕鼠胆酸升高、心肌酶谱变化以及胎鼠心肌超微结构的改变,提示这可能是ICP 导致突发不明原因死胎的原因之一。
Objective To explore the pathogenesis of intragepafic cholestasis of pregnancy（ICP）.Methods 40 pregnant rats during fourteenth day were selected and randomly divided into four groups. 1.2,2.5,5.0 mg/（kg·d） of estradioli benzoas （EB） were respctivily injected into rats of three test group to induce ICP. The four kinds of myocardial enzymes （AST, LDH, CK and CK-MB） of serum were tested in all pregnant rats. Same. time, the ultrastructure of cardiac myocytes of fetus was examined with dectromicroscope. Results The ulterastructural changes of cardiac myocytes in fetus rats included abnormal distribution of chrornatin, dilation and denaturalization of mitochondria, cytoplasmic vacuolizatian etc, and all of these changes depended on the dose of EB. In comparison with control group, TBA, AST and LDH of serum were higher in 2.5 mg/（kg·d） and 5.0mg/（kg·d） EB groups （ P 〈 0.05）. CK-MB of serum in 5.0mg/（kg·d） EB group was higher than that in control group （ P 〈 0. 05）. Conclusion The result suggests that these changes may be a cause induced stillbirth in ICP.
出处
《白求恩军医学院学报》
2006年第1期18-19,共2页
Journal of Bethune Military Medical College
关键词
肝内胆汁淤积
心肌酶
胆酸
胆红素
大鼠
Intrahepatie cholestasis
Cardiac enzymes
Bile acid
Bilimbin
Rat
