摘要
目的探讨高压氧(HBO)对缺血再灌注脑内兴奋性氨基酸水平的影响。方法将SD大鼠分为缺血再灌注组(I/R组)、高压氧处理组(HBO组)及假手术对照组(Sham-O组),以四动脉阻断法建立全脑缺血再灌注大鼠模型。动物缺血20min后分别再灌注6h、24h、48h和96h,各HBO组在再灌注后分别行不同次数的高压氧处理。取动物的全脑测定谷氨酸(Glu)和天门冬氨酸(Asp)含量。结果24h和48h HBO组Glu水平明显低于对应时间点的I/R组(P<0.01),且24h HBO组Asp含量也低于I/R组(P<0.05),而各时间点I/R组的Glu水平均高于Sham-O组(P<0.01)。结论在一定时间内进行高压氧处理可抑制由全脑缺血再灌注所诱导的兴奋性氨基酸的过度释放,这可能是高压氧治疗缺血再灌注脑损伤的机制之一。
Objective：To investigate the effect of hyperbaric oxygen （HBO） on the level of excitatory amino acid （EAA） in the brain after complete cerebral ischemia and reperfusion.nethod：SD rats were devided into three groups ： ischemia/reperfusion（I/R） group, hyperbaric oxygen treatment（HBO）group and sham-operated （Sham-O） group. The complete cerebral ischemia was induced by a four-vessel occlusion model. After 20 minutes of ischemia the animals were reperfused for 6, 24, 48 and 96 hours, respectively. The HBO group was subjected to different runs of hyperbaric oxygen treatment after reperfusion, respectively. The whole brain specimens of animals were obtained and the contents of glutamate （Glu） and aspartate （Asp） in the specimens were determined.Result：The levels of Glu of HBO groups at 24h and 48h were significantly lower than that of I/R groups of corresponding time（P≤0.01）. Furthermore, the levels of Asp of HBO group at 24h was also lower than that of I/R groups （ P≤0.05 ）, but the leves of Glu of I/R groups at different time points were higher than that of Sham-O group （P≤〈0.01）.Conclusion： The hyperbaric oxygen treatment can inhibit the overrelease of EAA induced by transient complete cerebral ischemia and reperfusion in the period of time. It may be one of mechanisms of HBO treatment brain damage following complete cerebral ischemia and reperfusion.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2006年第1期38-41,共4页
Chinese Journal of Rehabilitation Medicine
基金
北京市教委科技发展计划基金资助项目(00KJ-108)
关键词
高压氧
脑缺血
再灌注
兴奋性氨基酸
hyperbaric oxygen
brain ischemia
reperfusion
excitatory amino acid
