摘要
以发根农杆菌菌株A4转染已预培养1d的黄山药茎段后,共感染3d,其转化效果最佳;转化毛状根在无生长调节物质的MS培养基上培养可获得丛状芽,并发育成植株。
The perfect efficiency of genetic transformation of Dioscorea panthaica stem from pre-cultivation 1 d and co-cultivation 3 d, was got by the combination of strain A4 and Agrobacterium rhizogenes. Clustered shoots could grow from hairy roots in MS medium without growth regulators and then could form regenerated plants.
出处
《植物生理学通讯》
CSCD
北大核心
2005年第6期755-757,共3页
Plant Physiology Communications
关键词
黄山药
发根农杆菌
转化
再生植株
Dioscorea panthaica
Agrobacterium rhizogenes
transformation
plant regeneration
