摘要
目的观察心肺复苏(CPR)效果,并探讨窒息时间及CPR时间对大鼠自主循环恢复(ROSC)率和6h生存率的影响。方法采用呼气末夹闭气管窒息法,建立大鼠心脏停搏(A)模型。30只SpragueDawley(SD)大鼠随机分2组,窒息7min组(n=15),窒息9min组(n=15)。观察大鼠窒息及心肺复苏期间平均动脉压(MAP)、心电图(ECG)、心率(HR)和动脉血pH值的变化,比较各组的ROSC率和6h生存率。结果窒息7min组ROSC率和6h存活率与窒息9min组比较,差异无显著性(P>0.05);CPR时间<5min者6h生存率显著高于CPR时间>5min者(P<0.05);存活时间大于6h者CPR时间与存活时间小于6h者CPR时间比较有显著性差别(P<0.05)。结论该复苏模型制作简单,重复性好,可观察多个指标,能满足心肺复苏实验研究的需要;窒息时间选择应控制在7～9min以内;CPR时间是预测CPR成功率的重要指标。
Objective To observe the effect of cardiopulmonary resuscitation （CPR）, and to investigate the effect of aspyhxial time and CPR time on return of spontaneous circulation （ROSC） and 6-hour survival rate. Methods The animal model of cardiac arrest （CA） was made by clamping endotracheal tube at expiration. Thirty male Sprague Dawley （SD） rats were randomly divided into two groups： 7-minute asphyxiation group （ n = 15） and 9-minute asphyxiation group （ n = 15）. Mean arterial pressure （MAP）, electrocardiogram （ECG）, heart rate （HR） and arterial blood gas were monitored during asphyxia and CPR. The ROSC and 6-hour survival rate were compared between the two groups. Results Th.e ROSC rate and 6-hour survival rate in 7-minute asphyxiation group were not statistically different from those in 9-minute asphyxiation group （ P 〉 0.05）. 6-hour survival rate was higher in rats whose CPR 〈 5 minutes than that in rats whose CPR 〉 5 minutes （P 〈 0.05）. CPR in rats that survived more than 6 hours was shorter than that in rats surviving less than 6 hours （ P 〈 0.05）. Conclusion The model is easy to be duplicated Asphyxia time can be selected between 7 and 9 minutes. The CPR time is an important predictor of resuscitation success rate .
出处
《中华急诊医学杂志》
CAS
CSCD
2005年第10期814-817,共4页
Chinese Journal of Emergency Medicine
基金
温州市科技计划重点项目(Y2004A002)
温州市科技计划项目(S2002A020)
关键词
窒息法
大鼠
心脏骤停
动物模型
心肺复苏
Cardiopulmonary resuscitation （CPR）
Cardiac arrest （CA）
Asphyxia
Model
Rat
