摘要
目的：了解高压氧对免疫功能的影响。方法：高压氧作用后，摘眼球取血，对正常和荷瘤Ｂａｌｂ／Ｃ小鼠的ＲＢＣ－Ｃ３ｂ受体花环率，ＲＢＣ免疫复合物花环率及肿瘤细胞。淋巴细胞花环率进行了观察。结果：（１）高压氧处理组的ＲＢＣ－Ｃ３ｂ受体花环率明显高于对照组（Ｐ＝０．００７）；（２）肿瘤高压氧处理组的ＲＢＣ－Ｃ３６受体花环率明显高于单纯肿瘤接种组（Ｐ＝０．０２１）。（３）肿瘤高压氧处理组的肿瘤细胞－淋巴细胞花环率明显高于对照组（Ｐ＝０．００５）；（４）肿瘤高压氧处理组的肿瘤细胞：淋巴细胞花环率明显高于单纯高压氧处理组（Ｐ＝０．０３６）。（５）各组的ＲＢＣ－ＩＣ花环率无明显变化。结论：（１）压力为０．２ＭＰａ（２ＡＴＡ），氧浓度为８７％的高压氧不仅可以提高正常小鼠的免疫功能，而且可以提高荷瘤小鼠的免疫功能；（２）该条件的高压氧可使移植瘤引起的免疫功能低下程度得到减轻。
Aim:To study the effects of hyperbaric oxygen (HBO)on blood cell immune function. Methods:Twenty male balb/Cmice were randomly divided into 4(A,B,C,D)groups with each group five mice. Group A was the normal contrast. Group B was exposed to HBO. Group C was transplanted with S-180 cells,Group D was both exposed to HBO and transplanted with S-180 cells. The HBO(87%O2,0.2 MPa)treatment was once a day for twenty days. The blood samples were got by enucle- ating eyes of the mice. The RBC-C3b recel,tor,RBC-IC and tumour cell-lymphocyte rosette rates of all the mice were observed.Result:The RBC-C3b receptor rosette rates in group B were higher than in group A(p =0.007)and that of group D were higher than in group C(P=0.021)respectively. The tu- mour cell-lymphocyte rosette rate in group D was higher than in group A(p=0.005)and group B(P= 0.036).The RBC-IC rosette rates in various groups were not chngted significantly(P>0.05).Conclu- sion:HBO treatments in this experiment could increase the immune function not only in the normal mice but also in the tumor-bearing mice;besides the immune deficiency caused by transplant tumour in the mice could be lessened.
关键词
高压氧治疗
红细胞
免疫功能
Hyperbaric oxygen Immunity Receptor,C3b Neolpasm
