摘要
目的:研究大鼠脂肪基质干细胞(adipose-derived stem cells,ADSCs)分离培养的方法,探讨大鼠脂肪基质干细胞在体外向成骨细胞分化的能力。方法:取成年Sprague-Dawley大鼠腹股沟处脂肪组织,胶原酶消化分离,接种于自制基础培养基中分离传代。于基础培养基中传至第二代时改换诱导培养基,诱导向成骨细胞分化,培养2～4周,用碱性磷酸酶染色和Von Kossa染色鉴定成骨细胞分化能力。结果:大鼠脂肪中能够分离培养出生长旺盛的脂肪基质干细胞;经向成骨细胞诱导培养后,碱性磷酸酶染色和Von Kossa染色阳性,证实细胞能够分化为成骨细胞。结论:大鼠脂肪组织可分离培养出脂肪基质干细胞,生物学特性与骨髓基质干细胞(mes-enchymal stem cells,MSCs)相似,能够向成骨细胞分化,有望成为组织工程的种子细胞来源。
Objective：To study the method of isolating and culturing stem cells from rat adipose tissue and to determine the osteogenic capacity of adipose-derived stromal cells （ADSCs） harvest from rat in vitro. Method：ADSCs isolated from rat inguinal fat pads were digested with eollagenase.After primary culture in control medium anti expansion to the 2nd passages,the cells were incubated in induced medium for 2-4 weeks to induce osteogenesis.Osteogenic differentiation was tested by alkaline phosphatase and Von Kossa staining.Result：ADSCs could be isolated from rat adipose tissue,after osteogenesis induction,the result for alkaline phosphatase and Von Kossa staining was positive.Conclusion：Adipose-derived stromal cells can be isolated from rat adipnse tissue,their biological characteristics are analogy to mesenchymal stromal cells （MSCs）,and have the potential to differentiate into osteogenic lineage.It may be a source for tissue engineering.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2005年第9期553-555,i0001,共4页
Chinese Journal of Spine and Spinal Cord
