摘要
纸层电泳和凝胶层析表明魔芋(Amorphophallus tivieri)细胞壁多糖平均分子量为72000。酸水解只有甘露糖。Smith 降解只给出乙酰甘油醇,甲基化分析为2、3、4,6-四-O-甲基-1,5-二乙酰甘露糖醇,3、4、6-三-O-甲基-1、2、5-三乙酰甘露糖醇和3、6-二-O-甲基-1、2、4、5-四乙酰甘露糖醇,三者比例为1.08∶32∶1。
The cell-wall polysaccharide from Amorphophallus rivieri was found to be a mannan of MW.72000.The anomeric configuration was determined from the specific rotation and IR spec- trum of the polysaccharide.Smith degradation gave glycerol acetate only.The complete me- thylation followed by hydrolysis and acetylation gave 2,3,4,6-tetra-O-methyl-l,5-di-acetylmanni- tol,3,4,6-tri-O-methyl-1,2,5-tri-acetylmannitoi and 3,6-di-O-methyl-1,2,4,5-tetra-acetylmannitol in the ratio of 1.08:32:1.Based on all of these results,the structure assigned to the repeating unit of the polysaccharide was given.
关键词
魔芋
细胞壁多糖
甘露聚糖
Amorphophallus rivieri
Cell-wall polysaccharide
Mannan
