摘要
本实验用兔抗牛LH(bLH)血清,^(125)Ⅰ-标记牛LH(^(125)Ⅰ-bLH)建立猪LH(pLH)的异源放射免疫分析法。bLH、pLH的标准曲线及猪血清稀释曲线平行。此方法的特异性强,与猪促卵泡素(pFSH)的交叉反应小于0.5％;灵敏度为0.015～0.020ng/管;猪血清添加pLH的回收率为96.8±3.8％;测定内变异系数为3.3％(n=14),测定间变异系数为5.5％(n-5),用本方法测定,注射LRH-A_210分钟后猪血清LH的含量显著提高(P<0.01)。75、95、115、135、155日龄后备母香猪(n=7)血清中LH含量依次为1.50±0.59,1.54±0.42,1.76±0.30,1.41±0.17,1.88±0.30ng/ml;发情时母香猪(n=2)血清中LH含量为7.07±2.
A highly sensitive double antibody radioimmunoassay for measuring serum LH in the pig has been developed by using rabbit antibovine LH serum and radioiodinated bovine LH. In this heterologous system , the dose-response curve of bovine LH was parallel to that of porcine LH. Purified porcine FSH ( NIU-FSH-P2 ) showed cross-reactivity of less than 0.5%. The sensitivity of the assay was found to be 0.015~0.020ng per tube for USDA-pLH-B-1. The recovery of added porcine LH to Serum was 96.8±3.8%. The coefficients of variation within a single assay and between assay were 3.3% and 5.5% respectively. By employing this assay, the concentrations of LH ( mean?s.d.)in the sera of female Xiang Zhu pig on day 75, 95, 115, 135, 155 after birth ( n-7 ) and at es-trus ( n-2 ) were measured as 1.50±0.59, 1.54±0.42,1.76±0.30 , 1.41± 0.17,1.88±0.30 and 7.07±2.73 ng/ml. Ten minutes after 5μg LRH-A2 injection, the LH concentration in gilt serum was significantly elevated ( 0.52 v.s. 4.38 ng/ml ) .
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1989年第2期102-106,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
猪促黄体素
牛促黄体素
放射免疫
Porcine luteinizing hormone, Radioimmunoassay, Het- erologous, Bovine luteinizing hormone
